Anti-ECH1 antibody [3B7CF2AC8] (ab123450)

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Overview

  • Product name
    Anti-ECH1 antibody [3B7CF2AC8]
    See all ECH1 primary antibodies
  • Description
    Mouse monoclonal [3B7CF2AC8] to ECH1
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WB, IP, In-Cell ELISA, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Purified Human liver mitochondria.

  • Positive control
    • HDFn cells; Human, Rat and Cow heart homogenates; Human liver homogenate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab123450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
  • Pathway
    Lipid metabolism; fatty acid beta-oxidation.
  • Sequence similarities
    Belongs to the enoyl-CoA hydratase/isomerase family.
  • Cellular localization
    Mitochondrion. Peroxisome.
  • Information by UniProt
  • Database links
    • Alternative names
      • 4)-dienoyl-CoA isomerase antibody
      • 5)-Delta(2 antibody
      • Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
      • Delta(3 5) Delta(2 4) dienoyl CoA isomerase mitochondrial antibody
      • Delta(3 antibody
      • Delta3 5 delta2 4 dienoyl CoA isomerase antibody
      • Dienoyl CoA isomerase antibody
      • ECH 1 antibody
      • Ech1 antibody
      • ECH1_HUMAN antibody
      • Enoyl Coenzyme A hydratase 1 antibody
      • Enoyl Coenzyme A hydratase 1 peroxisomal antibody
      • HPXEL antibody
      • mitochondrial antibody
      • MS1034 antibody
      • Peroxisomal enoyl CoA hydratase 1 antibody
      • Peroxisomal enoyl coenzyme A hydratase like protein antibody
      see all

    Images

    • Western blot - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      Western blot - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      All lanes : Anti-ECH1 antibody [3B7CF2AC8] (ab123450) at 1 µg/ml

      Lane 1 : Human heart homogenate
      Lane 2 : Human liver homogenate
      Lane 3 : Rat heart homogenate
      Lane 4 : Cow heart homogenate

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      All lanes : Goat-anti-Mouse HRP

      Predicted band size: 36 kDa

    • Immunocytochemistry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      Immunocytochemistry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      ab123450 at 1µg/ml staining ECH1 in 4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized HDFn cells by Immunocytochemistry followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).
    • Flow Cytometry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      Flow Cytometry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      ab123450 at 1µg/ml staining ECH1 in HDFn cells by Flow Cytometry (blue) or no primary antibody control (red).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)

      IHC image of ECH1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab123450, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    ab123450 has not yet been referenced specifically in any publications.

    Publishing research using ab123450? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Please send a testing discount for testing reactivity with mouse samples by western blotting.

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    Answer

    I am very pleased to hear you would like to accept our offer and test ab123450 on western blots of mouse samples. This code will give you one free antibody before the expiration date. To redeem this offer, please submit an Abreview of WB mouse reactivity and include this code in the “Additional Comments” section so we know the Abreview is for this promotion.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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