Product nameAnti-ECH1 antibody [3B7CF2AC8]
See all ECH1 primary antibodies
DescriptionMouse monoclonal [3B7CF2AC8] to ECH1
Tested applicationsSuitable for: IHC-P, WB, IP, In-Cell ELISA, ICC, Flow Cytmore details
Species reactivityReacts with: Rat, Cow, Human
Purified Human liver mitochondria.
- HDFn cells; Human, Rat and Cow heart homogenates; Human liver homogenate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline
Concentration information loading...
Purification notesPurity is near homogeneity as judged by SDS-PAGE. ab123450 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab123450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.|
|IP||Use at an assay dependent concentration.|
|In-Cell ELISA||Use a concentration of 1 µg/ml.|
|ICC||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionIsomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
PathwayLipid metabolism; fatty acid beta-oxidation.
Sequence similaritiesBelongs to the enoyl-CoA hydratase/isomerase family.
Cellular localizationMitochondrion. Peroxisome.
- Information by UniProt
- 4)-dienoyl-CoA isomerase antibody
- 5)-Delta(2 antibody
- Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
All lanes : Anti-ECH1 antibody [3B7CF2AC8] (ab123450) at 1 µg/ml
Lane 1 : Human heart homogenate
Lane 2 : Human liver homogenate
Lane 3 : Rat heart homogenate
Lane 4 : Cow heart homogenate
Lysates/proteins at 10 µg/ml per lane.
All lanes : Goat-anti-Mouse HRP
Predicted band size: 36 kDa
ab123450 at 1µg/ml staining ECH1 in 4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized HDFn cells by Immunocytochemistry followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).
ab123450 at 1µg/ml staining ECH1 in HDFn cells by Flow Cytometry (blue) or no primary antibody control (red).
IHC image of ECH1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab123450, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab123450 has not yet been referenced specifically in any publications.