Overview

  • Product name

  • Description

    Rabbit polyclonal to ECH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 97-328 of Human ECH1 (Uniprot ID: Q13011).

  • Positive control

    • Recombinant Human ECH1 protein (ab101154) can be used as a positive control in WB. A549, HepG2 and Mouse brain whole cell lysates; HeLa cells. IHC-P (FFPE): Human Colon (Normal) tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
  • Pathway

    Lipid metabolism; fatty acid beta-oxidation.
  • Sequence similarities

    Belongs to the enoyl-CoA hydratase/isomerase family.
  • Cellular localization

    Mitochondrion. Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4)-dienoyl-CoA isomerase antibody
    • 5)-Delta(2 antibody
    • Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
    • Delta(3 5) Delta(2 4) dienoyl CoA isomerase mitochondrial antibody
    • Delta(3 antibody
    • Delta3 5 delta2 4 dienoyl CoA isomerase antibody
    • Dienoyl CoA isomerase antibody
    • ECH 1 antibody
    • Ech1 antibody
    • ECH1_HUMAN antibody
    • Enoyl Coenzyme A hydratase 1 antibody
    • Enoyl Coenzyme A hydratase 1 peroxisomal antibody
    • HPXEL antibody
    • mitochondrial antibody
    • MS1034 antibody
    • Peroxisomal enoyl CoA hydratase 1 antibody
    • Peroxisomal enoyl coenzyme A hydratase like protein antibody
    see all

Images

  • All lanes : Anti-ECH1 antibody (ab153720) at 1/2000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : ECH1 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 36 kDa

  • Anti-ECH1 antibody (ab153720) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 36 kDa



    10% SDS PAGE
  • IHC image of ECH1 staining in Human Colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab153720, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Anti-ECH1 antibody (ab153720) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 36 kDa



    10% SDS PAGE
  • Confocal immunofluorescent analysis of methanol-fixed HeLa cells, labeling ECH1 with ab153720 at 1/500 dilution (green). Alpha-tubulin filaments labeled in red.

References

ab153720 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart (Embryonic))
Permeabilization
Yes - Tween-20
Specification
Heart (Embryonic)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 29 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 19 2016

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