Product nameAnti-ECH1 antibody [EPR15449(B)] - C-terminal
See all ECH1 primary antibodies
DescriptionRabbit monoclonal [EPR15449(B)] to ECH1 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ECH1 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q13011
- Human fetal liver, A549, Jurkat and HeLa lysates; Human endometrium and kidney tissues; MCF-7 cells; Jurkat cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab189255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 36 kDa).|
|IHC-P||1/250 - 1/500.
Perform heat mediated antigen retrieval with EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionIsomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
PathwayLipid metabolism; fatty acid beta-oxidation.
Sequence similaritiesBelongs to the enoyl-CoA hydratase/isomerase family.
Cellular localizationMitochondrion. Peroxisome.
- Information by UniProt
- 4)-dienoyl-CoA isomerase antibody
- 5)-Delta(2 antibody
- Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
All lanes : Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/50000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : A549 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Immunhistochemical analysis of paraffin-embedded human endometrium tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunhistochemical analysis of paraffin-embedded human kidney tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunfluorescent analysis of 100% methanol(-20℃)-fixed MCF-7 cells labeling ECH1 with ab189254 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling ECH1 with ab189255 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of ECH1 in human fetal liver lysate immunoprecipitated using ab189254 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
ab189255 has not yet been referenced specifically in any publications.