Overview

  • Product name
    Anti-ECH1 antibody [EPR15449(B)] - C-terminal
    See all ECH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR15449(B)] to ECH1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ECH1 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q13011

  • Positive control
    • Human fetal liver, A549, Jurkat and HeLa lysates; Human endometrium and kidney tissues; MCF-7 cells; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab189255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/250 - 1/500.

Perform heat mediated antigen retrieval with EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF 1/100 - 1/250.
IP 1/60.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
  • Pathway
    Lipid metabolism; fatty acid beta-oxidation.
  • Sequence similarities
    Belongs to the enoyl-CoA hydratase/isomerase family.
  • Cellular localization
    Mitochondrion. Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4)-dienoyl-CoA isomerase antibody
    • 5)-Delta(2 antibody
    • Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
    • Delta(3 5) Delta(2 4) dienoyl CoA isomerase mitochondrial antibody
    • Delta(3 antibody
    • Delta3 5 delta2 4 dienoyl CoA isomerase antibody
    • Dienoyl CoA isomerase antibody
    • ECH 1 antibody
    • Ech1 antibody
    • ECH1_HUMAN antibody
    • Enoyl Coenzyme A hydratase 1 antibody
    • Enoyl Coenzyme A hydratase 1 peroxisomal antibody
    • HPXEL antibody
    • mitochondrial antibody
    • MS1034 antibody
    • Peroxisomal enoyl CoA hydratase 1 antibody
    • Peroxisomal enoyl coenzyme A hydratase like protein antibody
    see all

Images

  • All lanes : Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/50000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : A549 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 33 kDa
    why is the actual band size different from the predicted?

  • Immunhistochemical analysis of paraffin-embedded human endometrium tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunhistochemical analysis of paraffin-embedded human kidney tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunfluorescent analysis of 100% methanol(-20℃)-fixed MCF-7 cells labeling ECH1 with ab189254 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling ECH1 with ab189255 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Western blot analysis of ECH1 in human fetal liver lysate immunoprecipitated using ab189254 at 1/50 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

ab189255 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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