Overview

  • Product name

  • Description

    Rabbit polyclonal to ECHS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human ECHS1 (internal sequence). The exact sequence is proprietary.
    Database link: P30084

  • Positive control

    • WB: Mouse brain tissue whole cell lysate; HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Mouse heart, ovary and liver tissue. ICC/IF: A431 and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 31 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway.
  • Cellular localization

    Mitochondrion matrix.
  • Database links

  • Alternative names

    • C80529 antibody
    • EC 4.2.1.17 antibody
    • ECHS 1 antibody
    • Enoyl CoA hydratase 1 antibody
    • Enoyl CoA hydratase mitochondrial antibody
    • Enoyl CoA hydratase short chain 1 mitochondrial antibody
    • Enoyl Coenzyme A hydratase short chain 1 antibody
    • Enoyl Coenzyme A hydratase short chain 1 mitochondrial antibody
    • Mitochondrial short chain enoyl coenzyme A hydratase 1 antibody
    • OTTHUMP00000020811 antibody
    • SCEH antibody
    • Short chain enoyl CoA hydratase antibody
    see all

Images

  • A431 (human epidermoid carcinoma cell line) cells stained for ECHS1 (green) using ab228631 at 1/200 dilution in ICC/IF. Cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 minutes.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded mouse liver tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.

  • Anti-ECHS1 antibody (ab228631) at 1/1000 dilution + Mouse brain tissue whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 31 kDa



    12% SDS-PAGE

  • All lanes : Anti-ECHS1 antibody (ab228631) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 31 kDa



    12% SDS-PAGE

  • ECHS1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg ab228631. Western blot was performed from the immunoprecipitate using ab228631.

    Lane 1: HEK-293T whole cell extract (Input). 

    Lane 2: Control IgG instead of ab228631 in HEK-293T whole cell extract. 

    Lane 3: ab228631 IP in HEK-293T whole cell extract.

  • Paraffin-embedded mouse heart tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse ovary tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse liver tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ECHS1 (green) using ab228631 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.

    Blue: Hoechst 33342 staining.

References

ab228631 has not yet been referenced specifically in any publications.

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