Anti-ECHS1 antibody [EPR11784(B)] Recombinant (ab170108)

Product name

Anti-ECHS1 antibody [EPR11784(B)]
See all ECHS1 primary antibodies
Description

Rabbit monoclonal [EPR11784(B)] to ECHS1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human ECHS1 aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: P30084
Positive control
- Fetal liver and HeLa lysates; Human cardiac muscle and liver tissues; HeLa cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Our Abpromise guarantee covers the use of ab170108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P		1/100 - 1/250.
Flow Cyt		1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application

Abreviews

Notes

WB

1/1000 - 1/10000. Predicted molecular weight: 31 kDa.

IHC-P

1/100 - 1/250.

Flow Cyt

1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for ICC or IP.

Relevance

ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway.
Cellular localization

Mitochondrion matrix.
Database links
- Entrez Gene: 1892 Human
- Entrez Gene: 93747 Mouse
- Entrez Gene: 140547 Rat
- Omim: 602292 Human
- SwissProt: P30084 Human
- SwissProt: Q8BH95 Mouse
- SwissProt: P14604 Rat
- Unigene: 76394 Human
- Unigene: 24452 Mouse
- Unigene: 6847 Rat
see all
Alternative names
- C80529 antibody
- EC 4.2.1.17 antibody
- ECHS 1 antibody
- Enoyl CoA hydratase 1 antibody
- Enoyl CoA hydratase mitochondrial antibody
- Enoyl CoA hydratase short chain 1 mitochondrial antibody
- Enoyl Coenzyme A hydratase short chain 1 antibody
- Enoyl Coenzyme A hydratase short chain 1 mitochondrial antibody
- Mitochondrial short chain enoyl coenzyme A hydratase 1 antibody
- OTTHUMP00000020811 antibody
- SCEH antibody
- Short chain enoyl CoA hydratase antibody
see all

Flow Cytometry - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

Flow cytometric analysis of permeabilized HeLa cells labeling ECHS1 with ab170108 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ECHS1 with ab170108 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling ECHS1 with ab170108 at 1/100 dilution.
Western blot - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

All lanes : Anti-ECHS1 antibody [EPR11784(B)] (ab170108) at 1/1000 dilution

Lane 1 : Fetal liver lysate
Lane 2 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 31 kDa

ab170108 has not yet been referenced specifically in any publications.

Publishing research using ab170108? Please let us know so that we can cite the reference in this datasheet.

Anti-ECHS1 antibody [EPR11784(B)] (ab170108)