RecombinantRabMAb

Recombinant Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

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Flow Cytometry - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
  • Western blot - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
  • Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11784(B)] to ECHS1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ECHS1 antibody [EPR11784(B)]
    See all ECHS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11784(B)] to ECHS1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ECHS1 aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P30084

  • Positive control

    • Fetal liver and HeLa lysates; Human cardiac muscle and liver tissues; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab170108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Relevance

      ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway.

    • Cellular localization

      Mitochondrion matrix.

    • Database links

      see all

    • Alternative names

      • C80529 antibody
      • EC 4.2.1.17 antibody
      • ECHS 1 antibody
      • Enoyl CoA hydratase 1 antibody
      • Enoyl CoA hydratase mitochondrial antibody
      • Enoyl CoA hydratase short chain 1 mitochondrial antibody
      • Enoyl Coenzyme A hydratase short chain 1 antibody
      • Enoyl Coenzyme A hydratase short chain 1 mitochondrial antibody
      • Mitochondrial short chain enoyl coenzyme A hydratase 1 antibody
      • OTTHUMP00000020811 antibody
      • SCEH antibody
      • Short chain enoyl CoA hydratase antibody
      see all

    Images

    • Flow Cytometry - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Flow Cytometry - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

      Flow cytometric analysis of permeabilized HeLa cells labeling ECHS1 with ab170108 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ECHS1 with ab170108 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

      Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling ECHS1 with ab170108 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Western blot - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      All lanes : Anti-ECHS1 antibody [EPR11784(B)] (ab170108) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    • Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

    References (1)

    Publishing research using ab170108? Please let us know so that we can cite the reference in this datasheet.

    ab170108 has been referenced in 1 publication.

    • Bhopale KK  et al. Proteomic Profiling of Liver and Plasma in Chronic Ethanol Feeding Model of Hepatic Alcohol Dehydrogenase-Deficient Deer Mice. Alcohol Clin Exp Res 41:1675-1685 (2017). PubMed: 28792616

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