Recombinant
RabMAb

Recombinant Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

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Overview

  • Product name

    Anti-ECHS1 antibody [EPR11784(B)]
    See all ECHS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11784(B)] to ECHS1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ECHS1 aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P30084

  • Positive control

    • Fetal liver and HeLa lysates; Human cardiac muscle and liver tissues; HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Relevance

      ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway.

    • Cellular localization

      Mitochondrion matrix.

    • Database links

      see all

    • Alternative names

      • C80529 antibody
      • EC 4.2.1.17 antibody
      • ECHS 1 antibody
      • Enoyl CoA hydratase 1 antibody
      • Enoyl CoA hydratase mitochondrial antibody
      • Enoyl CoA hydratase short chain 1 mitochondrial antibody
      • Enoyl Coenzyme A hydratase short chain 1 antibody
      • Enoyl Coenzyme A hydratase short chain 1 mitochondrial antibody
      • Mitochondrial short chain enoyl coenzyme A hydratase 1 antibody
      • OTTHUMP00000020811 antibody
      • SCEH antibody
      • Short chain enoyl CoA hydratase antibody
      see all

    Images

    • Flow Cytometry - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Flow Cytometry - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

      Flow cytometric analysis of permeabilized HeLa cells labeling ECHS1 with ab170108 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ECHS1 with ab170108 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

      Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling ECHS1 with ab170108 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Western blot - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      All lanes : Anti-ECHS1 antibody [EPR11784(B)] (ab170108) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    References

    This product has been referenced in:

    • Bhopale KK  et al. Proteomic Profiling of Liver and Plasma in Chronic Ethanol Feeding Model of Hepatic Alcohol Dehydrogenase-Deficient Deer Mice. Alcohol Clin Exp Res 41:1675-1685 (2017). Read more (PubMed: 28792616) »
    See 1 Publication for this product

    Publishing research using ab170108? Please let us know so that we can cite the reference in this datasheet.

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