Recombinant
RabMAb

Anti-ECHS1 antibody [EPR11785] (ab174312)

Overview

  • Product name
    Anti-ECHS1 antibody [EPR11785]
    See all ECHS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11785] to ECHS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ECHS1 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P30084

  • Positive control
    • MCF7, HeLa, 293T, HepG2, Human fetal liver and fetal muscle lysates; Human heart tissue; Human liver tissue; MCF7 cell
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Predicted molecular weight: 31 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance
      ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway.
    • Cellular localization
      Mitochondrion matrix.
    • Database links
    • Alternative names
      • C80529 antibody
      • EC 4.2.1.17 antibody
      • ECHS 1 antibody
      • Enoyl CoA hydratase 1 antibody
      • Enoyl CoA hydratase mitochondrial antibody
      • Enoyl CoA hydratase short chain 1 mitochondrial antibody
      • Enoyl Coenzyme A hydratase short chain 1 antibody
      • Enoyl Coenzyme A hydratase short chain 1 mitochondrial antibody
      • Mitochondrial short chain enoyl coenzyme A hydratase 1 antibody
      • OTTHUMP00000020811 antibody
      • SCEH antibody
      • Short chain enoyl CoA hydratase antibody
      see all

    Images

    • All lanes : Anti-ECHS1 antibody [EPR11785] (ab174312) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Human fetal liver lysate
      Lane 6 : Human fetal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 31 kDa

    • Immunofluorescent staining of MCF7 cells labeling ECHS1 with ab174312 at 1/250 dilution (red). DAPI nuclear staining (blue).

    • Immunohistochemical analysis of paraffin embedded Human liver tissue labaling ECHS1 with ab174312 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ECHS1 with ab174312 at 1/100 dilution.

    References

    ab174312 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174312.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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