Product nameECL Extra - LumiBlue™
ECL Extra - LumiBlue™ (ab270229) is a non-isotopic, luminol-based chemiluminescence substrate, designed for the chemiluminescent detection of immobilized proteins and immobilized nucleic acids conjugated with horseradish peroxidase (HRP).
This product is manufactured by Expedeon, an Abcam company. Expedeon product code ECLA0250 was previously called LumiBlue™ ECL Extra - 250ml and is the same as the 250 mL size of this product. Expedeon product code ECLA0500 was previously called LumiBlue™ ECL Extra - 500ml and is the same as the 500 mL size.
The peroxidase-catalyzed oxidation of luminol produces a weak flash of light at 425 nm. The incorporation of an electron transfer mediator into the buffer forces the flash signal into a glow and greatly improves the analytical characteristics of the reaction in terms of increased signal intensity and duration. Recent works have shown that, by addition of a suitable acylation catalyst, a further large increase in light output is observed.
ECL Extra - LumiBlue™ (ab270229) has high signal intensity, good signal duration and is most suitable for detection of medium-abundance proteins.
The solution is sensitive enough to detect 500fg of protein per band and has a signal duration of up to 12 hours. The working solution is stable for up to three days after activation, allowing convenient experiments spanning multiple days and minimising waste.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Tested applicationsSuitable for: WBmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 250 ml 500 ml Luminol/enhancer solution 1 unit 1 unit Peroxide solution 1 unit 1 unit
Our Abpromise guarantee covers the use of ab270229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
ab270229 has not yet been referenced specifically in any publications.