Product nameECL Extreme - LumiBlue™
ECL Extreme - LumiBlue™ (ab270517) is a non-isotopic, luminol-based chemiluminescence substrate, designed for the chemiluminescent detection of immobilized proteins and immobilized nucleic acids conjugated with horseradish peroxidase (HRP).
This product is manufactured by Expedeon, an Abcam company. Expedeon product code ECLM0100 was previously called LumiBlue™ ECL Extreme - 100ml and is the same as the 100 mL size of this product. Expedeon product code ECLM0200 was previously called LumiBlue™ ECL Extreme - 200ml and is the same as the 200 mL size.
The peroxidase-catalyzed oxidation of luminol produces a weak flash of light at 425 nm. The incorporation of an electron transfer mediator into the buffer forces the flash signal into a glow and greatly improves the analytical characteristics of the reaction in terms of increased signal intensity and duration. Recent works have shown that, by addition of a suitable acylation catalyst, a further large increase in light output is observed.
ECL Extreme - LumiBlue™ (ab270517) is sensitive enough to detect approximately 50fg of protein per band and has a signal duration of up to eight hours. ECL Extreme has a chemiluminescence intensity 150 times greater than standard ECL substrates and gives bright, clear bands at super low protein concentrations.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Tested applicationsSuitable for: WBmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 100 ml 200 ml Luminol/enhancer solution 1 unit 1 unit Peroxide solution 1 unit 1 unit
Our Abpromise guarantee covers the use of ab270517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
ab270517 has not yet been referenced specifically in any publications.