Overview

  • Product name
    ECL Substrate Kit (High Sensitivity)
    See all ECL Substrate kits
  • Product overview

    High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. The high sensitivity ECL substrate produces a strong signal with very low background. Additionally, the ECL signal is long lasting, allowing repeated exposures without fear of losing data.


    Our ECL kits include our popular ECL Substrate Kit ab65623 and our high sensitivity ECL substrate kits:
    - this kit (High Sensitivity ECL Substrate Kit ab133406) to detect 23pg-187ng of protein per band
    - Very High Sensitivity ECL Substrate Kit ab133408 to detect 4.6pg-4.7ng of protein per band
    - Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per band 


    Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.


    This product was previously called Optiblot ECL Detect Kit (23pg-187ng).

  • Notes

    Use:
    - 200ml kit for 2000cm2 membrane
    - 500ml kit for 5000cm2 membrane

    The primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1/5000 - 1/20,000, with a typical secondary antibody dilution range of 1/20,000 - 1/100,000. Some optimisation may be required.

     

  • Tested applications
    Suitable for: WBmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab133406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Images

  • Anti-Transferrin antibody (ab1223) at 1/25000 dilution. Lanes 1-14 Transferrin protein (ab91435), Loading dilution (Lanes 1-8): 12.5, 6.2, 3.1, 1.6, 0.8, 0.3, 0.19µg protein.

     

    Secondary

    HRP conjugated polyclonal to Rabbit IgG (ab97080) at 1/50000 developed using Optiblot ECL Detect and Vendor A ECL subtrate.

  • Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) and with a rabbit secondary (ab97080). The blot was developed with 20ml of Optiblot ECL Detect.

Protocols

References

This product has been referenced in:
  • Ozsoy AZ  et al. Altered expression of p97/Valosin containing protein and impaired autophagy in preeclamptic human placenta. Placenta 67:45-53 (2018). Read more (PubMed: 29941173) »
  • Kostarnoy AV  et al. Receptor Mincle promotes skin allergies and is capable of recognizing cholesterol sulfate. Proc Natl Acad Sci U S A 114:E2758-E2765 (2017). WB . Read more (PubMed: 28292894) »
See all 5 Publications for this product

Customer reviews and Q&As

Filter by Ratings

1-2 of 2 Abreviews

Abreviews
The solution is highly sensitive, generating signal very fast (beta actin came in 20-30 sec, using the optiblot ECL detection solution developed in chem doc instrument).

Abcam user community

Verified customer

Submitted Nov 09 2015

Visualization of Western blots

Good Good 4/5 (Ease of Use)
Abreviews
This abcam optiblot detect kit gives signal very fast like that of the west dura. For my protein of interest, Signal appeared between 50sec to 1.3mins in chemidoc, B-actin comes even faster (30-40sec). The concentration of the total protein loaded was 40ug).

Abcam user community

Verified customer

Submitted Aug 07 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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