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Inquiry: I would like to know if the ECl Ultra detect kit is compatible with fluorescent imagers, such as the Typhoon imagers. Furthermore, I am currently using the ECL2 (formerly ECL Plus) detection kit, and I would like a small sample in order to compare to what I am currently using. Is that possible?
Asked on Oct 24 2012
Since you need a suitable ECL for imagers, I suggest to use ab133408 (Optiblot ECL Max Detect Kit (4.6pg-4.7ng)) which also corresponds with the ECL plus you are using at the moment.
ab133408 has been validated for CCD/laser use and generates a linear and quantitative signal that is very stable.
https://www.abcam.com/ECL-Substrate-Kit-Very-High-Sensitivity-ab133408.html (or use the following: https://www.abcam.com/ECL-Substrate-Kit-Very-High-Sensitivity-ab133408.html).
Please note that stripping the membrane is not recommended.
ab133409 (Optiblot ECL Ultra Detect Kit (1.2pg-2ng)) is also suitable but is much more sensitive. This means that with average expression levels of proteins the dilution factor of the antibodies has to be newly optimised (higher dilution factor).
Unfortunately, we do not have samples sizes of our product since this is logistically not feasible with over 95000 products.
But I would like to confirm that our products are covered by our Abpromise for six month after purchase.
Answered on Oct 24 2012