• Product name

    ECL Western Blotting Substrate Kit
  • Product overview

    The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.

    Our ECL kits include this kit ab65623 and our high sensitivity ECL substrate kits:
    - High Sensitivity ECL Substrate Kit ab133406 to detect 23pg-187ng of protein per band
    - Very High Sensitivity ECL Substrate Kit ab133408 to detect 4.6pg-4.7ng of protein per band
    - Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per band 

    Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.


  • Notes

    Reagents provided are sufficient for 400 cm2 of membrane.

  • Tested applications

    Suitable for: WBmore details



Our Abpromise guarantee covers the use of ab65623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.


  • ECL Western Blotting Substrate


This product has been referenced in:

  • Ju C  et al. Regulatory effect of miR-421 on humeral fracture and heterotopic ossification in elderly patients. Exp Ther Med 17:1903-1911 (2019). Read more (PubMed: 30783467) »
  • Yang C  et al. miRNA-155 expression and role in pathogenesis in spinal tuberculosis-induced intervertebral disc destruction. Exp Ther Med 17:3239-3246 (2019). Read more (PubMed: 30936999) »
See all 16 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

I love this product. It works super in many of my western blots, it carries a very low background and strong signals. It started to work 30 seconds after adding to the membrane. Even when I had to delay the exposure for about 30min, the signals are still very strong. Its catalog number was ab65628, don't know why abcam changed it to ab65623, but the price stays the same.

Abcam user community

Verified customer

Submitted Dec 04 2017


We have not specifically tested this Anti-CD9 antibody [EPR2949] (ab92726) as a marker of urinary exosomes but I can confirm that it can be used in western blot on mouse, rat, and human samples. Additionally, I have done a Google search and found that CD9 is indeed an exosome marker therefore it can be used as you mentioned. For more details about CD9 please see the publication below.

Can urinary exosomes act as treatment response markers in prostate cancer? Journal of Translational Medicine 2009, 7:4


If you are also looking for a second antibody and detection reagents to go with this ab92726, here are some recommendation.

https://www.abcam.co.jp/donkey-polyclonal-secondary-antibody-to-rabbit-igg-h-l-hrp-ab97064.html [1mg; 28,000 yen; WB dilution 1/5000 - 1/50000]


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Thank you for contacting us.

I am sorry do you need protcol or datasheet? The datasheet is attached.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry.

ab65623 is a good choice for developing western blots which use HRP conjugated secondary antibodies.

Did your customer want one antibody that would react with the Fc region of IgA, IgG and IgM or did they want 3 antibodies, one of which would react with IgA, one that would react with IgG and one that would react with IgM?

I checked our catalog and we do have one antibody, https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Human-IgG-IgM-IgA-H-L-HRP-ab102420.htmlthat will react with humanIgA, IgG and IgM but it is not specific for the Fc portion.

I hope this helps. I'll be happy to answer any further questions.

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Thank you for your enquiry. I can confirm that both products (ab5801 and ab65623) can be used for Western blot application, however ab65623 can be applied for more test (50). Your customer may be interested in our very new range of WB reagents (Optiblot) and further information can found at this site: www.abcam.com/optiblot I am happy to arrange a discount if they would like to try this product. I hope this helps and if I can assist further, please do not hesitate to contact me.

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