Anti-Eco1 antibody - C-terminal (ab211475)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Eco1 antibody - C-terminal -
Description
Rabbit polyclonal to Eco1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Eco1 aa 661-687 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Sequence:EYPDGRIIMVLPEDPKYALKKVDEIR
Database link: Q5FWF5 -
Positive control
- Human kidney and liver tissues; 293 cell lysates; Mouse spleen tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab211475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. | |
| WB | 1/1000. Predicted molecular weight: 95 kDa. |
Target
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Function
Acetyltransferase required for the establishment of sister chromatid cohesion and couple the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3. -
Tissue specificity
Widely expressed. Expressed in heart, brain, liver, placenta, lung, kidney and pancreas. Highly expressed in muscle. -
Sequence similarities
Belongs to the acetyltransferase family. GCN5 subfamily. -
Domain
The N-terminal region seems to be responsible for the association with chromosomes, thus excluding any involvement of the Zn finger in this process. -
Post-translational
modificationsPhosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Chromosome. Nuclear at interphase, associated with chromosomes during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 114799 Human
- Entrez Gene: 77805 Mouse
- Omim: 609674 Human
- SwissProt: Q5FWF5 Human
- SwissProt: Q69Z69 Mouse
- Unigene: 464733 Human
- Unigene: 210996 Mouse
- Unigene: 450684 Mouse
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Alternative names
- A930014I12Rik antibody
- CTF antibody
- CTF7 homolog 1 antibody
see all
Images
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Western blot - Anti-Eco1 antibody - C-terminal (ab211475)Anti-Eco1 antibody - C-terminal (ab211475) at 1/1000 dilution + Mouse spleen tissue lysate at 35 µg
Predicted band size: 95 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eco1 antibody - C-terminal (ab211475)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labelling Eco1 with ab211475 at 1/100 dilution.
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Western blot - Anti-Eco1 antibody - C-terminal (ab211475)Anti-Eco1 antibody - C-terminal (ab211475) at 1/1000 dilution + 293 cell lysate at 35 µg
Predicted band size: 95 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eco1 antibody - C-terminal (ab211475)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelling Eco1 with ab211475 at 1/100 dilution.
Datasheets and documents
References
ab211475 has not yet been referenced specifically in any publications.
