Anti-Eco1 antibody - C-terminal (ab211475)
Key features and details
- Rabbit polyclonal to Eco1 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Eco1 antibody - C-terminal -
Description
Rabbit polyclonal to Eco1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Eco1 aa 661-687 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Sequence:EYPDGRIIMVLPEDPKYALKKVDEIR
Database link: Q5FWF5 -
Positive control
- Human kidney and liver tissues; 293 cell lysates; Mouse spleen tissue lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab211475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. | |
| WB | 1/1000. Predicted molecular weight: 95 kDa. |
Target
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Function
Acetyltransferase required for the establishment of sister chromatid cohesion and couple the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3. -
Tissue specificity
Widely expressed. Expressed in heart, brain, liver, placenta, lung, kidney and pancreas. Highly expressed in muscle. -
Sequence similarities
Belongs to the acetyltransferase family. GCN5 subfamily. -
Domain
The N-terminal region seems to be responsible for the association with chromosomes, thus excluding any involvement of the Zn finger in this process. -
Post-translational
modificationsPhosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Chromosome. Nuclear at interphase, associated with chromosomes during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 114799 Human
- Entrez Gene: 77805 Mouse
- Omim: 609674 Human
- SwissProt: Q5FWF5 Human
- SwissProt: Q69Z69 Mouse
- Unigene: 464733 Human
- Unigene: 210996 Mouse
- Unigene: 450684 Mouse
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Alternative names
- A930014I12Rik antibody
- CTF antibody
- CTF7 homolog 1 antibody
see all
Images
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Western blot - Anti-Eco1 antibody - C-terminal (ab211475)Anti-Eco1 antibody - C-terminal (ab211475) at 1/1000 dilution + Mouse spleen tissue lysate at 35 µg
Predicted band size: 95 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eco1 antibody - C-terminal (ab211475)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labelling Eco1 with ab211475 at 1/100 dilution.
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Western blot - Anti-Eco1 antibody - C-terminal (ab211475)Anti-Eco1 antibody - C-terminal (ab211475) at 1/1000 dilution + 293 cell lysate at 35 µg
Predicted band size: 95 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eco1 antibody - C-terminal (ab211475)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelling Eco1 with ab211475 at 1/100 dilution.
Protocols
Datasheets and documents
References (0)
ab211475 has not yet been referenced specifically in any publications.
