Overview

  • Product name

    Anti-ECT2 antibody - N-terminal
    See all ECT2 primary antibodies
  • Description

    Rabbit polyclonal to ECT2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-44 of Human ECT2 (Uniprot: Q9H8V3).

  • Positive control

    • Human A549 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8 .

Target

  • Relevance

    The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
  • Cellular localization

    ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis
  • Database links

  • Alternative names

    • ECT 2 antibody
    • ECT2 protein antibody
    • Epithelial cell transforming sequence 2 antibody
    • Epithelial cell transforming sequence 2 oncogene antibody
    • Epithelial cell transforming sequence 2 oncogene protein antibody
    • FLJ10461 antibody
    • MGC138291 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling ECT2 with ab151503 at 1/100 dilution.

References

This product has been referenced in:

  • Chen Z  et al. Role of Epithelial Cell Transforming Sequence 2 (ECT2) in Predicting Prognosis of Osteosarcoma. Med Sci Monit 23:3861-3868 (2017). Read more (PubMed: 28794404) »
See 1 Publication for this product

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