Overview

  • Product name

    Anti-EDAR antibody [EPR8020]
    See all EDAR primary antibodies
  • Description

    Rabbit monoclonal [EPR8020] to EDAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EDAR aa 300-400 (intracellular). The exact sequence is proprietary.

  • Positive control

    • Fetal lung, A431, MCF7, 293T, and A549 lysates; 293T cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Receptor for EDA isoform A1, but not for EDA isoform A2. Mediates the activation of NF-kappa-B and JNK. May promote caspase-independent cell death.
    • Tissue specificity

      Detected in fetal kidney, lung, skin and cultured neonatal epidermal keratinocytes. Not detected in lymphoblast and fibroblast cell lines.
    • Involvement in disease

      Defects in EDAR are a cause of ectodermal dysplasia anhidrotic (EDA) [MIM:224900]; also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands.
      Defects in EDAR are the cause of ectodermal dysplasia type 3 (ED3) [MIM:129490]; also known as ectodermal dysplasia hypohidrotic autosomal dominant or EDA3. ED3 is an autosomal dominant condition characterized by hypotrichosis, abnormal or missing teeth, and hypohidrosis due to the absence of sweat glands.
    • Sequence similarities

      Contains 1 death domain.
      Contains 3 TNFR-Cys repeats.
    • Developmental stage

      Found in craniofacial tissues from embryonic day 42-53. Expressed in fetal skin 11 and 15 weeks after gestation.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Anhidrotic ectodysplasin receptor 1 antibody
      • DL antibody
      • Downless (mouse) homolog antibody
      • Downless homolog antibody
      • Downless mouse homolog of antibody
      • ECTD10A antibody
      • ECTD10B antibody
      • Ectodermal dysplasia receptor antibody
      • Ectodysplasin 1 anhidrotic receptor antibody
      • Ectodysplasin A receptor antibody
      • Ectodysplasin A1 isoform receptor antibody
      • Ectodysplasin-A receptor antibody
      • ED 1R antibody
      • ED 3 antibody
      • ED 5 antibody
      • ED1R antibody
      • ED3 antibody
      • ED5 antibody
      • EDA 1R antibody
      • EDA 3 antibody
      • EDA A1 receptor antibody
      • EDA A1R antibody
      • EDA-A1 receptor antibody
      • EDA1R antibody
      • EDA3 antibody
      • Edar antibody
      • EDAR_HUMAN antibody
      • HRM1 antibody
      • Tumor necrosis factor receptor superfamily member EDAR antibody
      see all

    Images

    • All lanes : Anti-EDAR antibody [EPR8020] (ab137021) at 1/1000 dilution

      Lane 1 : Fetal lung lysate
      Lane 2 : A431 lysate
      Lane 3 : MCF7 lysate
      Lane 4 : 293T lysate
      Lane 5 : A549 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 49 kDa

    • Flow Cytometric analysis of permeabilized 293T cells labelling EDAR with ab137021 at 1/100 dilution (red). Rabbit IgG negative control is green.

    References

    This product has been referenced in:

    See 1 Publication for this product

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