Anti-EDC3 antibody (ab168851)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-EDC3 antibody -
Description
Rabbit polyclonal to EDC3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 25-75 of Human EDC3 (NP_079359.2).
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Positive control
- 293T, HeLa, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab168851 was affinity purified using an epitope specific to EDC3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab168851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 56 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Binds single-stranded RNA. In the process of mRNA degradation, may play a role in mRNA decapping. May play a role in spermiogenesis and oogenesis. -
Tissue specificity
Expressed in theca and granulosa cells in ovary, and in spermatids of the meiotic division part II and apical membrane of Sertoli cells in testis (at protein level). Also expressed in brain and mammary gland. -
Sequence similarities
Belongs to the EDC3 family.
Contains 1 DFDF domain.
Contains 1 YjeF N-terminal domain. -
Domain
The DFDF domain is unstructured by itself. It assumes a helical fold upon interaction with DDX6. -
Cellular localization
Cytoplasm > P-body. Processing bodies. - Information by UniProt
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Database links
- Entrez Gene: 80153 Human
- Entrez Gene: 353190 Mouse
- Omim: 609842 Human
- SwissProt: Q96F86 Human
- SwissProt: Q8K2D3 Mouse
- Unigene: 682454 Human
- Unigene: 96852 Human
- Unigene: 268104 Mouse
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Alternative names
- EDC 3 antibody
- edc3 antibody
- EDC3_HUMAN antibody
see all
Images
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Western blot - Anti-EDC3 antibody (ab168851)All lanes : Anti-EDC3 antibody (ab168851) at 0.1 µg
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-EDC3 antibody (ab168851)Detection of EDC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168851 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds
Datasheets and documents
References
This product has been referenced in:
- D'Lima NG et al. A human microprotein that interacts with the mRNA decapping complex. Nat Chem Biol 13:174-180 (2017). Read more (PubMed: 27918561) »
- Hubstenberger A et al. P-Body Purification Reveals the Condensation of Repressed mRNA Regulons. Mol Cell 68:144-157.e5 (2017). Read more (PubMed: 28965817) »
