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    edc3-antibody-ab168851.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Other
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Anti-EDC3 antibody (ab168851)

  • Datasheet
Submit a review Submit a question References (2)

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Western blot - Anti-EDC3 antibody (ab168851)
  • Immunoprecipitation - Anti-EDC3 antibody (ab168851)

Key features and details

  • Rabbit polyclonal to EDC3
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human EDC3 protein (ab132637)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-EDC3 antibody
  • Description

    Rabbit polyclonal to EDC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 25-75 of Human EDC3 (NP_079359.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab168851 was affinity purified using an epitope specific to EDC3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human EDC3 protein (ab132637)

Applications

Our Abpromise guarantee covers the use of ab168851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 56 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Binds single-stranded RNA. In the process of mRNA degradation, may play a role in mRNA decapping. May play a role in spermiogenesis and oogenesis.
  • Tissue specificity

    Expressed in theca and granulosa cells in ovary, and in spermatids of the meiotic division part II and apical membrane of Sertoli cells in testis (at protein level). Also expressed in brain and mammary gland.
  • Sequence similarities

    Belongs to the EDC3 family.
    Contains 1 DFDF domain.
    Contains 1 YjeF N-terminal domain.
  • Domain

    The DFDF domain is unstructured by itself. It assumes a helical fold upon interaction with DDX6.
  • Cellular localization

    Cytoplasm > P-body. Processing bodies.
  • Target information above from: UniProt accession Q96F86 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 80153 Human
    • Entrez Gene: 353190 Mouse
    • Omim: 609842 Human
    • SwissProt: Q96F86 Human
    • SwissProt: Q8K2D3 Mouse
    • Unigene: 682454 Human
    • Unigene: 96852 Human
    • Unigene: 268104 Mouse
    • Alternative names

      • EDC 3 antibody
      • edc3 antibody
      • EDC3_HUMAN antibody
      • enhancer of mRNA decapping 3 homolog (S. cerevisiae) antibody
      • Enhancer of mRNA decapping 3 homolog antibody
      • Enhancer of mRNA decapping 3, S. cerevisiae, homolog of antibody
      • Enhancer of mRNA decapping protein 3 antibody
      • Enhancer of mRNA-decapping protein 3 antibody
      • FLJ21128 antibody
      • FLJ31777 antibody
      • hYjeF_N2 antibody
      • hYjeF_N2-15q23 antibody
      • LSM 16 antibody
      • LSM16 antibody
      • LSM16 homolog (EDC3 S. cerevisiae) antibody
      • LSM16 homolog (EDC3, S. cerevisiae) antibody
      • LSM16 homolog antibody
      • YJDC antibody
      • YjeF domain containing antibody
      • YjeF domain containing protein 1 antibody
      • YjeF domain containing protein antibody
      • YjeF domain-containing protein 1 antibody
      • YjeF N-terminal domain-containing protein 2 antibody
      • YjeF_N2 antibody
      • YJEFN2 antibody
      see all

    Images

    • Western blot - Anti-EDC3 antibody (ab168851)
      Western blot - Anti-EDC3 antibody (ab168851)
      All lanes : Anti-EDC3 antibody (ab168851) at 0.1 µg

      Lane 1 : 293T whole cell lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : Jurkat whole cell lysate
      Lane 4 : TCMK-1 whole cell lysate
      Lane 5 : NIH3T3 whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 56 kDa


      Exposure time: 10 seconds
    • Immunoprecipitation - Anti-EDC3 antibody (ab168851)
      Immunoprecipitation - Anti-EDC3 antibody (ab168851)
      Detection of EDC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168851 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
      Detection: Chemiluminescence with an exposure time of 10 seconds

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab168851? Please let us know so that we can cite the reference in this datasheet.

    ab168851 has been referenced in 2 publications.

    • D'Lima NG  et al. A human microprotein that interacts with the mRNA decapping complex. Nat Chem Biol 13:174-180 (2017). PubMed: 27918561
    • Hubstenberger A  et al. P-Body Purification Reveals the Condensation of Repressed mRNA Regulons. Mol Cell 68:144-157.e5 (2017). PubMed: 28965817

    Customer reviews and Q&As

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