Product nameAnti-EDC3 antibody
DescriptionRabbit polyclonal to EDC3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
- 293T, HeLa, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab168851 was affinity purified using an epitope specific to EDC3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab168851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 56 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionBinds single-stranded RNA. In the process of mRNA degradation, may play a role in mRNA decapping. May play a role in spermiogenesis and oogenesis.
Tissue specificityExpressed in theca and granulosa cells in ovary, and in spermatids of the meiotic division part II and apical membrane of Sertoli cells in testis (at protein level). Also expressed in brain and mammary gland.
Sequence similaritiesBelongs to the EDC3 family.
Contains 1 DFDF domain.
Contains 1 YjeF N-terminal domain.
DomainThe DFDF domain is unstructured by itself. It assumes a helical fold upon interaction with DDX6.
Cellular localizationCytoplasm > P-body. Processing bodies.
- Information by UniProt
- EDC 3 antibody
- edc3 antibody
- EDC3_HUMAN antibody
All lanes : Anti-EDC3 antibody (ab168851) at 0.1 µg
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 10 seconds
Detection of EDC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168851 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds
This product has been referenced in:
- D'Lima NG et al. A human microprotein that interacts with the mRNA decapping complex. Nat Chem Biol 13:174-180 (2017). Read more (PubMed: 27918561) »
- Hubstenberger A et al. P-Body Purification Reveals the Condensation of Repressed mRNA Regulons. Mol Cell 68:144-157.e5 (2017). Read more (PubMed: 28965817) »