Key features and details
- Rabbit polyclonal to EDD
- Suitable for: IP, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
- Isotype: IgG
Product nameAnti-EDD antibody
See all EDD primary antibodies
DescriptionRabbit polyclonal to EDD
Tested applicationsSuitable for: IP, WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
Synthetic peptide corresponding to Human EDD aa 1900-2230.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab175810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 309 kDa.|
FunctionE3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
Tissue specificityWidely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 PABC domain.
Contains 1 UBR-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
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All lanes : Anti-EDD antibody (ab175810)
Lane 1 : Control si
Lane 2 : UBR5 si
Lane 3 : NIH 3T3 cell lysate at 40 µg
Lane 4 : PC12 cell lysate at 40 µg
Predicted band size: 309 kDa
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling EDD with ab175810 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Top - EDD, Middle - DAPI, Bottom - Merge.
Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling EDD with ab175810 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Top - 4X, Middle - 10X, Bottom - 20X.
Immunoprecipitation analysis of U2OS cells labelling EED with ab175810. Protein A beads were crosslinked to the serum.
ab175810 has not yet been referenced specifically in any publications.