Product nameAnti-EDD antibody
See all EDD primary antibodies
DescriptionGoat polyclonal to EDD
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Predicted to work with: Cow, Pig
General notesGenBank Accession Number – NP_056986. LocusLink ID - 51366 (human); 70790 (mouse); 117060 (rat). Gene Ontology terms - ubiquitin conjugating enzyme activity; ubiquitin-protein ligase activity; ubiquitin cycle; cell proliferation; protein modification; soluble fraction; nucleus; ligase activity.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab4376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).Can be blocked with Human EDD peptide (ab23029).|
|IP||Use at an assay dependent dilution.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. No results could not be obtained after antigen retrieval at pH6.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21148289|
FunctionE3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
Tissue specificityWidely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 PABC domain.
Contains 1 UBR-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 4432411E13Rik antibody
- AW549941 antibody
- C77315 antibody
Anti-EDD antibody (ab4376) at 1/500 dilution + MDA-MB-468 breast cancer cells (25µg)
Donkey anti-goat conjugated to HRP at 1/4000 dilution
Performed under reducing conditions.
Predicted band size: 309 kDa
Exposure time: 5 seconds
Developed using SuperBlock Blocking Buffer (pierce) and pico ECL kit (pierce) to reduce the backgound observed - please refer to abreview for further experimental details.
ab4376 (4µg/ml) staining of paraffin embedded Human Testis shows nuclear staining . Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.
This product has been referenced in:
- Hay-Koren A et al. The EDD E3 ubiquitin ligase ubiquitinates and up-regulates beta-catenin. Mol Biol Cell 22:399-411 (2011). WB ; Human . Read more (PubMed: 21118991) »
- Won JH et al. Phenotypic changes in mouse pancreatic stellate cell Ca2+ signaling events following activation in culture and in a disease model of pancreatitis. Mol Biol Cell 22:421-36 (2011). ICC/IF . Read more (PubMed: 21148289) »