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  1. Link

    edd-antibody-ab70311.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes Hect E3 Ligase
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Anti-EDD antibody (ab70311)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (9)

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Western blot - Anti-EDD antibody (ab70311)
  • Immunoprecipitation - Anti-EDD antibody (ab70311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)

Key features and details

  • Rabbit polyclonal to EDD
  • Suitable for: IHC-P, WB, IP, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-EDD antibody [EPR4841] (ab134089)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-EDD antibody
    See all EDD primary antibodies
  • Description

    Rabbit polyclonal to EDD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residues 600 and 650 of human EDD. (NP_056986.2 )

  • Positive control

    • HeLa and NIH3T3 whole cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70311 was affinity purified using an epitope specific to EDD immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Hect E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
1/2000 - 1/10000. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).
IP
Use at 2-10 µg/mg of lysate.
ICC/IF
Use at an assay dependent concentration.
Notes
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).
IP
Use at 2-10 µg/mg of lysate.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
  • Tissue specificity

    Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 PABC domain.
    Contains 1 UBR-type zinc finger.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O95071 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 515582 Cow
    • Entrez Gene: 51366 Human
    • Entrez Gene: 70790 Mouse
    • Entrez Gene: 100157625 Pig
    • Entrez Gene: 117060 Rat
    • Omim: 608413 Human
    • SwissProt: O95071 Human
    • SwissProt: Q80TP3 Mouse
    • SwissProt: Q62671 Rat
    • Unigene: 492445 Human
    • Unigene: 476840 Mouse
    • Unigene: 54812 Rat
    see all
  • Alternative names

    • 4432411E13Rik antibody
    • AW549941 antibody
    • C77315 antibody
    • D030042K14 antibody
    • DD5 antibody
    • E3 identified by differential display antibody
    • E3 ubiquitin protein ligase, HECT domain containing, 1 antibody
    • E3 ubiquitin-protein ligase antibody
    • E3 ubiquitin-protein ligase UBR5 antibody
    • EDD 1 antibody
    • EDD antibody
    • EDD1 antibody
    • FLJ11310 antibody
    • HECT domain-containing 1 antibody
    • hHYD antibody
    • HYD antibody
    • Hyperplastic discs protein homolog antibody
    • Hyperplastic discs, Drosophila, homolog of antibody
    • KIAA0896 antibody
    • MGC57263 antibody
    • mKIAA0896 antibody
    • Progestin induced protein antibody
    • Progestin-induced protein antibody
    • Rat100 antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin protein ligase E3 component n recognin 5 antibody
    • UBR5 antibody
    • UBR5_HUMAN antibody
    see all

Images

  • Western blot - Anti-EDD antibody (ab70311)
    Western blot - Anti-EDD antibody (ab70311)
    All lanes : Anti-EDD antibody (ab70311) at 0.4 µg/ml

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : LNCaP whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 309 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-EDD antibody (ab70311)
    Immunoprecipitation - Anti-EDD antibody (ab70311)

    Immunoprecipitation of HeLa cells using ab70311

    Lane 1: ab70311 current lot

    Lane 2: ab70311 previous lot

    Lane 3: Control IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)

    Immunohistochemistry analysis of formalin-fixed paraffin-embedded sections of mouse plasmacytoma staining EDD with ab70311 at 1/1000 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)

    Immunohistochemistry analysis of formalin-fixed paraffin-embedded sections of human lung carcinoma staining EDD with ab70311 at 1/1000 dilution

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (9)

Publishing research using ab70311? Please let us know so that we can cite the reference in this datasheet.

ab70311 has been referenced in 9 publications.

  • Wang K  et al. UBR5 regulates proliferation and radiosensitivity in human laryngeal carcinoma via the p38/MAPK signaling pathway. Oncol Rep 44:685-697 (2020). PubMed: 32468011
  • Duan Z  et al. TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein. Virulence 11:607-635 (2020). PubMed: 32420802
  • Khan MM  et al. Protein interactome of the Cancerous Inhibitor of protein phosphatase 2A (CIP2A) in Th17 cells. Curr Res Immunol 1:10-22 (2020). PubMed: 33817627
  • Ding F  et al. UBR5 oncogene as an indicator of poor prognosis in gastric cancer. Exp Ther Med 20:7 (2020). PubMed: 32934672
  • Xie Z  et al. Significance of the E3 ubiquitin protein UBR5 as an oncogene and a prognostic biomarker in colorectal cancer. Oncotarget 8:108079-108092 (2017). PubMed: 29296225
  • Flack JE  et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
  • Liao L  et al. E3 Ubiquitin Ligase UBR5 Drives the Growth and Metastasis of Triple-Negative Breast Cancer. Cancer Res 77:2090-2101 (2017). PubMed: 28330927
  • Bolt MJ  et al. Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-a protein levels and activity. Oncogene N/A:N/A (2014). Human . PubMed: 24441042
  • Morrison J  et al. Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling. PLoS Pathog 9:e1003265 (2013). WB ; Human . PubMed: 23555265

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-EDD antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (adipocyte)
Loading amount
5 µg
Specification
adipocyte
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted May 17 2011

Question

I am looking for a prestained protein standard that goes higher than 250 kD to use with this antibody. Do you have any recommendations?

Read More

Abcam community

Verified customer

Asked on Jan 31 2012

Answer

Thank you for contacting Abcam regarding ab70311.

Regarding the MW ladder to use with this antibody I have checked our catalog, but the ladders we carry only go as high as 245kDa (ab116029).

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Abcam Scientific Support

Answered on Jan 31 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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