Anti-EDD antibody (ab70311)
Key features and details
- Rabbit polyclonal to EDD
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-EDD antibody
See all EDD primary antibodies -
Description
Rabbit polyclonal to EDD -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
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Immunogen
Synthetic peptide corresponding to a region between residues 600 and 650 of human EDD. (NP_056986.2 )
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Positive control
- HeLa and NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70311 was affinity purified using an epitope specific to EDD immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).
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| IP |
Use at 2-10 µg/mg of lysate.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. -
Tissue specificity
Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 PABC domain.
Contains 1 UBR-type zinc finger. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 515582 Cow
- Entrez Gene: 51366 Human
- Entrez Gene: 70790 Mouse
- Entrez Gene: 100157625 Pig
- Entrez Gene: 117060 Rat
- Omim: 608413 Human
- SwissProt: O95071 Human
- SwissProt: Q80TP3 Mouse
see all -
Alternative names
- 4432411E13Rik antibody
- AW549941 antibody
- C77315 antibody
see all
Images
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Western blot - Anti-EDD antibody (ab70311)All lanes : Anti-EDD antibody (ab70311) at 0.4 µg/ml
Lane 1 : HEK293T whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : LNCaP whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 309 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-EDD antibody (ab70311)Immunoprecipitation of HeLa cells using ab70311
Lane 1: ab70311 current lot
Lane 2: ab70311 previous lot
Lane 3: Control IgG
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)Immunohistochemistry analysis of formalin-fixed paraffin-embedded sections of mouse plasmacytoma staining EDD with ab70311 at 1/1000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)Immunohistochemistry analysis of formalin-fixed paraffin-embedded sections of human lung carcinoma staining EDD with ab70311 at 1/1000 dilution
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab70311 has been referenced in 9 publications.
- Wang K et al. UBR5 regulates proliferation and radiosensitivity in human laryngeal carcinoma via the p38/MAPK signaling pathway. Oncol Rep 44:685-697 (2020). PubMed: 32468011
- Duan Z et al. TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein. Virulence 11:607-635 (2020). PubMed: 32420802
- Khan MM et al. Protein interactome of the Cancerous Inhibitor of protein phosphatase 2A (CIP2A) in Th17 cells. Curr Res Immunol 1:10-22 (2020). PubMed: 33817627
- Ding F et al. UBR5 oncogene as an indicator of poor prognosis in gastric cancer. Exp Ther Med 20:7 (2020). PubMed: 32934672
- Xie Z et al. Significance of the E3 ubiquitin protein UBR5 as an oncogene and a prognostic biomarker in colorectal cancer. Oncotarget 8:108079-108092 (2017). PubMed: 29296225
- Flack JE et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
- Liao L et al. E3 Ubiquitin Ligase UBR5 Drives the Growth and Metastasis of Triple-Negative Breast Cancer. Cancer Res 77:2090-2101 (2017). PubMed: 28330927
- Bolt MJ et al. Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-a protein levels and activity. Oncogene N/A:N/A (2014). Human . PubMed: 24441042
- Morrison J et al. Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling. PLoS Pathog 9:e1003265 (2013). WB ; Human . PubMed: 23555265