Anti-EDD antibody (ab70311)

Reviews (1)Q&A (1)References (5)

Key features and details

  • Rabbit polyclonal to EDD
  • Suitable for: IHC-P, ICC/IF, WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-EDD antibody
    See all EDD primary antibodies

  • Description

    Rabbit polyclonal to EDD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus

  • Immunogen

    Synthetic peptide corresponding to a region between residues 600 and 650 of human EDD. (NP_056986.2 )

  • Positive control

    • HeLa and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.

  • Tissue specificity

    Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 PABC domain.
    Contains 1 UBR-type zinc finger.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 4432411E13Rik antibody
    • AW549941 antibody
    • C77315 antibody
    • D030042K14 antibody
    • DD5 antibody
    • E3 identified by differential display antibody
    • E3 ubiquitin protein ligase, HECT domain containing, 1 antibody
    • E3 ubiquitin-protein ligase antibody
    • E3 ubiquitin-protein ligase UBR5 antibody
    • EDD 1 antibody
    • EDD antibody
    • EDD1 antibody
    • FLJ11310 antibody
    • HECT domain-containing 1 antibody
    • hHYD antibody
    • HYD antibody
    • Hyperplastic discs protein homolog antibody
    • Hyperplastic discs, Drosophila, homolog of antibody
    • KIAA0896 antibody
    • MGC57263 antibody
    • mKIAA0896 antibody
    • Progestin induced protein antibody
    • Progestin-induced protein antibody
    • Rat100 antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin protein ligase E3 component n recognin 5 antibody
    • UBR5 antibody
    • UBR5_HUMAN antibody
    see all

Images

  • Western blot - Anti-EDD antibody (ab70311)
    Western blot - Anti-EDD antibody (ab70311)
    All lanes : Anti-EDD antibody (ab70311) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 309 kDa
    Observed band size: 309 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling EDD with ab70311 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-EDD antibody (ab70311)
    Immunoprecipitation - Anti-EDD antibody (ab70311)
    Detection of Human EDD by Western Blot of Immunprecipitate. ab70311 at 3µg/ml staining EDD in HeLa whole cell lysates immunoprecipitated using ab70311 (1 mg/IP; 1/4 of IP loaded/lane).
  • Immunocytochemistry/ Immunofluorescence - Anti-EDD antibody (ab70311)
    Immunocytochemistry/ Immunofluorescence - Anti-EDD antibody (ab70311)
    ICC/IF image of ab70311 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70311, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab70311)
    IHC image of ab70311 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70311, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References (5)

Publishing research using ab70311? Please let us know so that we can cite the reference in this datasheet.

ab70311 has been referenced in 5 publications.

  • Xie Z  et al. Significance of the E3 ubiquitin protein UBR5 as an oncogene and a prognostic biomarker in colorectal cancer. Oncotarget 8:108079-108092 (2017). PubMed: 29296225
  • Flack JE  et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
  • Liao L  et al. E3 Ubiquitin Ligase UBR5 Drives the Growth and Metastasis of Triple-Negative Breast Cancer. Cancer Res 77:2090-2101 (2017). PubMed: 28330927
  • Bolt MJ  et al. Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-a protein levels and activity. Oncogene N/A:N/A (2014). Human . PubMed: 24441042
  • Morrison J  et al. Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling. PLoS Pathog 9:e1003265 (2013). WB ; Human . PubMed: 23555265

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I am looking for a prestained protein standard that goes higher than 250 kD to use with this antibody. Do you have any recommendations?

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Answer

Thank you for contacting Abcam regarding ab70311.

Regarding the MW ladder to use with this antibody I have checked our catalog, but the ladders we carry only go as high as 245kDa (ab116029).

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Western blot abreview for Anti-EDD antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (adipocyte)
Loading amount
5 µg
Specification
adipocyte
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted May 17 2011

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