• Product name
  • Description
    Rabbit polyclonal to EDD
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residues 600 and 650 of human EDD. (NP_056986.2 )

  • Positive control
    • HeLa and NIH3T3 whole cell lysates.



Our Abpromise guarantee covers the use of ab70311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).
IP Use at 2-10 µg/mg of lysate.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
  • Tissue specificity
    Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 PABC domain.
    Contains 1 UBR-type zinc finger.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • 4432411E13Rik antibody
    • AW549941 antibody
    • C77315 antibody
    • D030042K14 antibody
    • DD5 antibody
    • E3 identified by differential display antibody
    • E3 ubiquitin protein ligase, HECT domain containing, 1 antibody
    • E3 ubiquitin-protein ligase antibody
    • E3 ubiquitin-protein ligase UBR5 antibody
    • EDD 1 antibody
    • EDD antibody
    • EDD1 antibody
    • FLJ11310 antibody
    • HECT domain-containing 1 antibody
    • hHYD antibody
    • HYD antibody
    • Hyperplastic discs protein homolog antibody
    • Hyperplastic discs, Drosophila, homolog of antibody
    • KIAA0896 antibody
    • MGC57263 antibody
    • mKIAA0896 antibody
    • Progestin induced protein antibody
    • Progestin-induced protein antibody
    • Rat100 antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin protein ligase E3 component n recognin 5 antibody
    • UBR5 antibody
    • UBR5_HUMAN antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling EDD with ab70311 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EDD antibody (ab70311) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 309 kDa
    Observed band size: 309 kDa

    Exposure time: 30 seconds
  • Detection of Human EDD by Western Blot of Immunprecipitate. ab70311 at 3µg/ml staining EDD in HeLa whole cell lysates immunoprecipitated using ab70311 (1 mg/IP; 1/4 of IP loaded/lane).
  • ICC/IF image of ab70311 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70311, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab70311 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70311, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Xie Z  et al. Significance of the E3 ubiquitin protein UBR5 as an oncogene and a prognostic biomarker in colorectal cancer. Oncotarget 8:108079-108092 (2017). Read more (PubMed: 29296225) »
  • Flack JE  et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). Read more (PubMed: 28689657) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting Abcam regarding ab70311.

Regarding the MW ladder to use with this antibody I have checked our catalog, but the ladders we carry only go as high as 245kDa (ab116029).

I hope this information is helpful. Ple...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (adipocyte)
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted May 17 2011


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