Overview

  • Product name

    Anti-EDD antibody - C-terminal
    See all EDD primary antibodies
  • Description

    Rabbit polyclonal to EDD - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment within Human EDD (C terminal). The exact sequence is proprietary.
    Database link: O95071

  • Positive control

    • WB: U-87 MG whole cell lysate and nuclear extract. ICC/IF: U-87 MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 309 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
  • Tissue specificity

    Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 PABC domain.
    Contains 1 UBR-type zinc finger.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4432411E13Rik antibody
    • AW549941 antibody
    • C77315 antibody
    • D030042K14 antibody
    • DD5 antibody
    • E3 identified by differential display antibody
    • E3 ubiquitin protein ligase, HECT domain containing, 1 antibody
    • E3 ubiquitin-protein ligase antibody
    • E3 ubiquitin-protein ligase UBR5 antibody
    • EDD 1 antibody
    • EDD antibody
    • EDD1 antibody
    • FLJ11310 antibody
    • HECT domain-containing 1 antibody
    • hHYD antibody
    • HYD antibody
    • Hyperplastic discs protein homolog antibody
    • Hyperplastic discs, Drosophila, homolog of antibody
    • KIAA0896 antibody
    • MGC57263 antibody
    • mKIAA0896 antibody
    • Progestin induced protein antibody
    • Progestin-induced protein antibody
    • Rat100 antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin protein ligase E3 component n recognin 5 antibody
    • UBR5 antibody
    • UBR5_HUMAN antibody
    see all

Images

  • All lanes : Anti-EDD antibody - C-terminal (ab226792) at 1/500 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 309 kDa

  • 4% paraformaldehyde-fixed, U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for EDD (green) using ab226792 at 1/500 dilution in ICC/IF.

    Alpha-Tubulin is detected with an anti-alpha-Tubulin antibody at 1/1000 dilution (red).

References

ab226792 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226792.
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