Overview

  • Product name

    Anti-EDD antibody [EPR4841]
    See all EDD primary antibodies
  • Description

    Rabbit monoclonal [EPR4841] to EDD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human EDD. The exact sequence is proprietary.

  • Positive control

    • SH-SY5Y, HeLa, and 293T cell lysates.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 300 kDa (predicted molecular weight: 309 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
    • Tissue specificity

      Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
      Contains 1 PABC domain.
      Contains 1 UBR-type zinc finger.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4432411E13Rik antibody
      • AW549941 antibody
      • C77315 antibody
      • D030042K14 antibody
      • DD5 antibody
      • E3 identified by differential display antibody
      • E3 ubiquitin protein ligase, HECT domain containing, 1 antibody
      • E3 ubiquitin-protein ligase antibody
      • E3 ubiquitin-protein ligase UBR5 antibody
      • EDD 1 antibody
      • EDD antibody
      • EDD1 antibody
      • FLJ11310 antibody
      • HECT domain-containing 1 antibody
      • hHYD antibody
      • HYD antibody
      • Hyperplastic discs protein homolog antibody
      • Hyperplastic discs, Drosophila, homolog of antibody
      • KIAA0896 antibody
      • MGC57263 antibody
      • mKIAA0896 antibody
      • Progestin induced protein antibody
      • Progestin-induced protein antibody
      • Rat100 antibody
      • Ubiquitin protein ligase antibody
      • Ubiquitin protein ligase E3 component n recognin 5 antibody
      • UBR5 antibody
      • UBR5_HUMAN antibody
      see all

    Images

    • All lanes : Anti-EDD antibody [EPR4841] (ab134089) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 309 kDa

    References

    This product has been referenced in:

    • Chen L  et al. Fasting-induced hormonal regulation of lysosomal function. Cell Res 27:748-763 (2017). Read more (PubMed: 28374748) »
    • Wang J  et al. Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in liver cancer. J Biol Chem 288:33667-81 (2013). WB ; Human . Read more (PubMed: 24089522) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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