Product nameAnti-EDD antibody [EPR4841]
See all EDD primary antibodies
DescriptionRabbit monoclonal [EPR4841] to EDD
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human EDD. The exact sequence is proprietary.
- SH-SY5Y, HeLa, and 293T cell lysates.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 300 kDa (predicted molecular weight: 309 kDa).|
FunctionE3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
Tissue specificityWidely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 PABC domain.
Contains 1 UBR-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 4432411E13Rik antibody
- AW549941 antibody
- C77315 antibody
ab134089 has been referenced in 2 publications.
- Chen L et al. Fasting-induced hormonal regulation of lysosomal function. Cell Res 27:748-763 (2017). PubMed: 28374748
- Wang J et al. Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in liver cancer. J Biol Chem 288:33667-81 (2013). WB ; Human . PubMed: 24089522