Product nameAnti-EDEM1 antibody - Carboxyterminal end
See all EDEM1 primary antibodies
DescriptionRabbit polyclonal to EDEM1 - Carboxyterminal end
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Mouse, Human
Synthetic peptide corresponding to C-terminal residues of human EDEM1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab55920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 74 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|ELISA||Use at an assay dependent dilution.|
RelevanceEDEM1 is directly involved in ER associated degradation (ERAD), and targets misfolded glycoproteins for degradation in an N glycan dependent manner. It lacks mannosidase activity. EDEM1 belongs to the glycosyl hydrolase 47 family.
Cellular localizationEndoplasmic reticulum membrane.
- EDEM 1 antibody
- EDEM antibody
- Endoplasmic reticulum degradation enhancing alpha mannosidase like protein antibody
Anti-EDEM1 antibody - Carboxyterminal end (ab55920) at 2 µg/ml + Hela Whole Cell Lysate - Staurosporine Treated (48hr, 500nM) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 74 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible cross reactivity)
ab55920 has not yet been referenced specifically in any publications.