Key features and details
- Rabbit polyclonal to EDF1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EDF1 antibody
DescriptionRabbit polyclonal to EDF1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
- 293T, HeLa, and Jurkat whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab174651 was affinity purified using an epitope specific to EDF1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab174651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 16 kDa.|
FunctionTranscriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
Tissue specificityExpressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues.
Sequence similaritiesContains 1 HTH cro/C1-type DNA-binding domain.
Developmental stageExpressed in fetal tissues. More abundant in kidney.
DomainThe IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein.
modificationsPhosphorylated (by PKA and PKC).
Cellular localizationCytoplasm. Nucleus. Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.
- Information by UniProt
- CAP19 antibody
- EDF-1 antibody
- edf1 antibody
All lanes : Anti-EDF1 antibody (ab174651) at 0.100000001490116 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 16 kDa
Exposure time: 3 minutes
Detection of EDF1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab174651 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab174651 was used at 1 µg/ml. Lane 2 represents control IgG IP.
ab174651 has been referenced in 1 publication.
- Nishioka K et al. Mbf1 ensures Polycomb silencing by protecting E(z) mRNA from degradation by Pacman. Development 145:N/A (2018). PubMed: 29523653