Overview

  • Product name
    Anti-EDG2 / LPA-1 antibody
    See all EDG2 / LPA-1 primary antibodies
  • Description
    Rabbit polyclonal to EDG2 / LPA-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Cow, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human EDG2/ LPA-1 aa 342-359.
    Sequence:

    DRSASSLNH TILAGVHSN

  • Positive control
    • For western blot, Mouse liver, A549 cell lysate, Rat Liver lysate.
  • General notes

    Previously labelled as EDG2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab23698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 18772233
WB Use a concentration of 2 - 5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 41 kDa).
ICC Use a concentration of 8 µg/ml.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
  • Tissue specificity
    Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5031439C20 antibody
    • AI326300 antibody
    • EDG 2 antibody
    • EDG2 antibody
    • Endothelial differentiation gene 2 antibody
    • Endothelial differentiation, lysophosphatidic acid G protein coupled receptor, 2 antibody
    • GPCR 26 antibody
    • Gpcr26 antibody
    • Gpcr91 antibody
    • GPR26 antibody
    • Kdt2 antibody
    • LPA 1 antibody
    • LPA receptor 1 antibody
    • LPA receptor EDG2 antibody
    • LPA-1 antibody
    • lpA1 antibody
    • Lpar1 antibody
    • LPAR1_HUMAN antibody
    • Lysophosphatidic acid receptor 1 antibody
    • Lysophosphatidic acid receptor Edg-2 antibody
    • Lysophosphatidic acid receptor EDG2 antibody
    • MGC105279 antibody
    • MGC29102 antibody
    • Mrec1.3 antibody
    • rec.1.3 antibody
    • Ventricular zone gene 1 antibody
    • vzg-1 antibody
    • VZG1 antibody
    see all

Images

  • ab123698 staining EDG2 / LPA-1 in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1% Triton-X, blocked with 8% serum for 60 minutes at 35°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in PBS) at 4°C for 18 hours. An Alexa Fluor® 555-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-EDG2 / LPA-1 antibody (ab23698)

    Lane 1 : Solubilised Mouse Liver at 50 µg
    Lane 2 : A549 Cell Lysate at 40 µg
    Lane 3 : Rat liver lysate at 40 µg

    Predicted band size: 41 kDa

References

This product has been referenced in:
  • Cao P  et al. Autocrine lysophosphatidic acid signaling activates ß-catenin and promotes lung allograft fibrosis. J Clin Invest 127:1517-1530 (2017). Read more (PubMed: 28240604) »
  • Zhuang Y  et al. MiR-338* suppresses fibrotic pathogenesis in pulmonary fibrosis through targeting LPA1. Am J Transl Res 8:3197-205 (2016). WB ; Mouse . Read more (PubMed: 27508041) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Cortical cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - 0.1% Triton X
Specification
Cortical cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 35°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 10 2013

Answer

The antibody is specific for EDG2(LPAR1). We do not find any significant similarity of the peptide antigen used for LPAR1 antibody production to residues within the LPAR2 protein. The antibody should not detect EDG4 (LPAR2). I hope this is helpful. Please feel free to contact me with any further technical questions.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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