Product nameAnti-EDG2 / LPA-1 antibody
See all EDG2 / LPA-1 primary antibodies
DescriptionRabbit polyclonal to EDG2 / LPA-1
Tested applicationsSuitable for: IHC-Fr, WB, ICC, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Cow, Human
Predicted to work with: Xenopus laevis
- For western blot, Mouse liver, A549 cell lysate, Rat Liver lysate.
Previously labelled as EDG2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 30% Glycerol, 0.1% BSA, Tris buffered saline, pH 7.4
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab23698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 18772233|
|WB||Use a concentration of 2 - 5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 41 kDa).|
|ICC||Use a concentration of 8 µg/ml.|
|IHC-FoFr||Use at an assay dependent concentration.|
FunctionReceptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
Tissue specificityExpressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
- Information by UniProt
- 5031439C20 antibody
- AI326300 antibody
- EDG 2 antibody
ab123698 staining EDG2 / LPA-1 in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1% Triton-X, blocked with 8% serum for 60 minutes at 35°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in PBS) at 4°C for 18 hours. An Alexa Fluor® 555-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
All lanes : Anti-EDG2 / LPA-1 antibody (ab23698)
Lane 1 : Solubilised Mouse Liver at 50 µg
Lane 2 : A549 Cell Lysate at 40 µg
Lane 3 : Rat liver lysate at 40 µg
Predicted band size: 41 kDa
This product has been referenced in:
- Cao P et al. Autocrine lysophosphatidic acid signaling activates ß-catenin and promotes lung allograft fibrosis. J Clin Invest 127:1517-1530 (2017). Read more (PubMed: 28240604) »
- Zhuang Y et al. MiR-338* suppresses fibrotic pathogenesis in pulmonary fibrosis through targeting LPA1. Am J Transl Res 8:3197-205 (2016). WB ; Mouse . Read more (PubMed: 27508041) »