Recombinant
RabMAb

Anti-EDG2 / LPA-1 antibody [EPR9710] (ab166903)

Overview

  • Product name
    Anti-EDG2 / LPA-1 antibody [EPR9710]
    See all EDG2 / LPA-1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9710] to EDG2 / LPA-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EDG2/ LPA-1. The exact sequence is proprietary.
    Database link: Q92633
    (Peptide available as ab184693)

  • Positive control
    • Jurkat, HeLa, Caco 2 and A375 cell lysates, paraffin-embedded Human colon tissue, paraffin-embedded Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 39 kDa.Can be blocked with EDG2 peptide (ab184693).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
    • Tissue specificity
      Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization
      Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5031439C20 antibody
      • AI326300 antibody
      • EDG 2 antibody
      • EDG2 antibody
      • Endothelial differentiation gene 2 antibody
      • Endothelial differentiation, lysophosphatidic acid G protein coupled receptor, 2 antibody
      • GPCR 26 antibody
      • Gpcr26 antibody
      • Gpcr91 antibody
      • GPR26 antibody
      • Kdt2 antibody
      • LPA 1 antibody
      • LPA receptor 1 antibody
      • LPA receptor EDG2 antibody
      • LPA-1 antibody
      • lpA1 antibody
      • Lpar1 antibody
      • LPAR1_HUMAN antibody
      • Lysophosphatidic acid receptor 1 antibody
      • Lysophosphatidic acid receptor Edg-2 antibody
      • Lysophosphatidic acid receptor EDG2 antibody
      • MGC105279 antibody
      • MGC29102 antibody
      • Mrec1.3 antibody
      • rec.1.3 antibody
      • Ventricular zone gene 1 antibody
      • vzg-1 antibody
      • VZG1 antibody
      see all

    Images

    • All lanes : Anti-EDG2 / LPA-1 antibody [EPR9710] (ab166903) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Caco 2 cell lysate
      Lane 4 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP IgG antibody at 1/2000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EDG2 using ab166903 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EDG2 using ab166903 at 1/100 dilution.
    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166903 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:
    • Zhang G  et al. ATX-LPA axis facilitates estrogen-induced endometrial cancer cell proliferation via MAPK/ERK signaling pathway. Mol Med Rep 17:4245-4252 (2018). Read more (PubMed: 29328374) »
    • Xu M  et al. EDG2 enhanced the progression of hepatocellular carcinoma by LPA/PI3K/AKT/ mTOR signaling. Oncotarget 8:66154-66168 (2017). Read more (PubMed: 29029500) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Purified protein (BEL-7404)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    50 µg
    Treatment
    80 µM actinomycin for 24hrs
    Specification
    BEL-7404
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. guoqing zhu

    Verified customer

    Submitted Jan 26 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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