Product nameEDG2 / LPA-1 overexpression 293T lysate (whole cell)
ab94195 is a 293T cell transfected lysate in which Human EDG2 / LPA-1 has been transiently over-expressed using a pCMV-EDG2 / LPA-1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation. Tissue specificity: Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes. Similarity: Belongs to the G-protein coupled receptor 1 family.
Our Abpromise guarantee covers the use of ab94195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94195 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-EDG2 /LPA-1 antibody (ab89628) at 1/500 dilution
Lane 1 :
EDG2 / LPA-1 overexpression 293T lysate (whole cell) (ab94195)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94195 has not yet been referenced specifically in any publications.