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    edg2-lpa-1-antibody-epr9710-ab166903.pdf

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RecombinantRabMAb

Recombinant Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

  • Datasheet
Reviews (1) Submit a question References (9)

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Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
  • Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
  • ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
  • Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9710] to EDG2 /LPA-1
  • Suitable for: ELISA, WB, IHC-P, IP
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
Product image
Recombinant Human EDG2 /LPA-1 protein (ab152354)

View more associated products

Overview

  • Product name

    Anti-EDG2 /LPA-1 antibody [EPR9710]
    See all EDG2 /LPA-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9710] to EDG2 /LPA-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Synthetic peptide within Human EDG2/LPA-1. The exact sequence is proprietary.
    Database link: Q92633
    (Peptide available as ab184693)

  • Positive control

    • Jurkat, HeLa, Caco 2 and A375 cell lysates, paraffin-embedded Human colon tissue, paraffin-embedded Human kidney tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9710
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human EDG2 /LPA-1 protein (ab152354)
  • Related Products

    • Recombinant Human EDG2 /LPA-1 protein (ab152354)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Notes
ELISA
Use at an assay dependent concentration.
WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
  • Tissue specificity

    Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
  • Target information above from: UniProt accession Q92633 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1902 Human
    • Omim: 602282 Human
    • SwissProt: Q92633 Human
    • Unigene: 126667 Human
    • Alternative names

      • 5031439C20 antibody
      • AI326300 antibody
      • EDG 2 antibody
      • EDG2 antibody
      • Endothelial differentiation gene 2 antibody
      • Endothelial differentiation, lysophosphatidic acid G protein coupled receptor, 2 antibody
      • GPCR 26 antibody
      • Gpcr26 antibody
      • Gpcr91 antibody
      • GPR26 antibody
      • Kdt2 antibody
      • LPA 1 antibody
      • LPA receptor 1 antibody
      • LPA receptor EDG2 antibody
      • LPA-1 antibody
      • lpA1 antibody
      • Lpar1 antibody
      • LPAR1_HUMAN antibody
      • Lysophosphatidic acid receptor 1 antibody
      • Lysophosphatidic acid receptor Edg-2 antibody
      • Lysophosphatidic acid receptor EDG2 antibody
      • MGC105279 antibody
      • MGC29102 antibody
      • Mrec1.3 antibody
      • rec.1.3 antibody
      • Ventricular zone gene 1 antibody
      • vzg-1 antibody
      • VZG1 antibody
      see all

    Images

    • Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      All lanes : Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Caco 2 cell lysate
      Lane 4 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP IgG antibody at 1/2000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166903 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
    • ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

      ELISA analysis of Human EDG2/LPA-1 recombinant protein at 250 ng/mL with ab166903. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

    • Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (9)

    Publishing research using ab166903? Please let us know so that we can cite the reference in this datasheet.

    ab166903 has been referenced in 9 publications.

    • Wu D  et al. EZH2 promotes the expression of LPA1 by mediating microRNA-139 promoter methylation to accelerate the development of ovarian cancer. Cancer Cell Int 20:551 (2020). PubMed: 33292225
    • Akoume MY  et al. A Differential Hypofunctionality of Gai Proteins Occurs in Adolescent Idiopathic Scoliosis and Correlates with the Risk of Disease Progression. Sci Rep 9:10074 (2019). PubMed: 31296888
    • Cha YJ & Koo JS Expression of Autotaxin?Lysophosphatidate Signaling-Related Proteins in Breast Cancer with Adipose Stroma. Int J Mol Sci 20:N/A (2019). PubMed: 31035435
    • Shim SJ  et al. The expressions of autotaxin-lysophosphatidate signaling-related proteins in metastatic breast cancer. Int J Clin Exp Pathol 12:2920-2930 (2019). PubMed: 31934128
    • Chabowski DS  et al. Lysophosphatidic acid acts on LPA1 receptor to increase H2 O2 during flow-induced dilation in human adipose arterioles. Br J Pharmacol 175:4266-4280 (2018). PubMed: 30153326
    • Zhang G  et al. ATX-LPA axis facilitates estrogen-induced endometrial cancer cell proliferation via MAPK/ERK signaling pathway. Mol Med Rep 17:4245-4252 (2018). PubMed: 29328374
    • Xu M  et al. EDG2 enhanced the progression of hepatocellular carcinoma by LPA/PI3K/AKT/ mTOR signaling. Oncotarget 8:66154-66168 (2017). PubMed: 29029500
    • Hopkins MM  et al. Positive and Negative Cross-Talk between Lysophosphatidic Acid Receptor 1, Free Fatty Acid Receptor 4, and Epidermal Growth Factor Receptor in Human Prostate Cancer Cells. J Pharmacol Exp Ther 359:124-33 (2016). PubMed: 27474750
    • Qiao Y  et al. High Glucose Stimulates Tumorigenesis in Hepatocellular Carcinoma Cells Through AGER-Dependent O-GlcNAcylation of c-Jun. Diabetes 65:619-32 (2016). PubMed: 26825459

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-EDG2 / LPA-1 antibody [EPR9710]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (BEL-7404)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    50 µg
    Treatment
    80 µM actinomycin for 24hrs
    Specification
    BEL-7404
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. guoqing zhu

    Verified customer

    Submitted Jan 26 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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