Recombinant Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
![Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-165262-anti-edg2-lpa-1-antibody-epr9710-western-blot.jpg "Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9710] to EDG2 /LPA-1
- Suitable for: ELISA, WB, IHC-P, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-EDG2 /LPA-1 antibody [EPR9710]
See all EDG2 /LPA-1 primary antibodies -
Description
Rabbit monoclonal [EPR9710] to EDG2 /LPA-1 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
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Positive control
- Jurkat, HeLa, Caco 2 and A375 cell lysates, paraffin-embedded Human colon tissue, paraffin-embedded Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9710 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
1/10 - 1/100. |
Target
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Function
Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation. -
Tissue specificity
Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles. - Information by UniProt
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Database links
- Entrez Gene: 1902 Human
- Omim: 602282 Human
- SwissProt: Q92633 Human
- Unigene: 126667 Human
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Alternative names
- 5031439C20 antibody
- AI326300 antibody
- EDG 2 antibody
see all
Images
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Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)All lanes : Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP IgG antibody at 1/2000 dilution
Predicted band size: 39 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-165261-anti-edg2-lpa-1-antibody-epr9710-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-165260-anti-edg2-lpa-1-antibody-epr9710-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-165259-anti-edg2-lpa-1-antibody-epr9710-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166903 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
![ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-7-anti-edg2-lpa-1-antibody-epr9710-elisa-human-edg2lpa-1.jpg)
ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)ELISA analysis of Human EDG2/LPA-1 recombinant protein at 250 ng/mL with ab166903. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-5-benefits-of-recombinant-antibodies.png)
Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab166903 has been referenced in 9 publications.
- Wu D et al. EZH2 promotes the expression of LPA1 by mediating microRNA-139 promoter methylation to accelerate the development of ovarian cancer. Cancer Cell Int 20:551 (2020). PubMed: 33292225
- Akoume MY et al. A Differential Hypofunctionality of Gai Proteins Occurs in Adolescent Idiopathic Scoliosis and Correlates with the Risk of Disease Progression. Sci Rep 9:10074 (2019). PubMed: 31296888
- Cha YJ & Koo JS Expression of Autotaxin?Lysophosphatidate Signaling-Related Proteins in Breast Cancer with Adipose Stroma. Int J Mol Sci 20:N/A (2019). PubMed: 31035435
- Shim SJ et al. The expressions of autotaxin-lysophosphatidate signaling-related proteins in metastatic breast cancer. Int J Clin Exp Pathol 12:2920-2930 (2019). PubMed: 31934128
- Chabowski DS et al. Lysophosphatidic acid acts on LPA1 receptor to increase H2 O2 during flow-induced dilation in human adipose arterioles. Br J Pharmacol 175:4266-4280 (2018). PubMed: 30153326
- Zhang G et al. ATX-LPA axis facilitates estrogen-induced endometrial cancer cell proliferation via MAPK/ERK signaling pathway. Mol Med Rep 17:4245-4252 (2018). PubMed: 29328374
- Xu M et al. EDG2 enhanced the progression of hepatocellular carcinoma by LPA/PI3K/AKT/ mTOR signaling. Oncotarget 8:66154-66168 (2017). PubMed: 29029500
- Hopkins MM et al. Positive and Negative Cross-Talk between Lysophosphatidic Acid Receptor 1, Free Fatty Acid Receptor 4, and Epidermal Growth Factor Receptor in Human Prostate Cancer Cells. J Pharmacol Exp Ther 359:124-33 (2016). PubMed: 27474750
- Qiao Y et al. High Glucose Stimulates Tumorigenesis in Hepatocellular Carcinoma Cells Through AGER-Dependent O-GlcNAcylation of c-Jun. Diabetes 65:619-32 (2016). PubMed: 26825459
