Recombinant
RabMAb

Recombinant Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

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Overview

  • Product name

    Anti-EDG2 /LPA-1 antibody [EPR9710]
    See all EDG2 /LPA-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9710] to EDG2 /LPA-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human EDG2/LPA-1. The exact sequence is proprietary.
    Database link: Q92633
    (Peptide available as ab184693)

  • Positive control

    • Jurkat, HeLa, Caco 2 and A375 cell lysates, paraffin-embedded Human colon tissue, paraffin-embedded Human kidney tissue.

  • General notes

    Previously labelled as EDG2.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab166903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 39 kDa.Can be blocked with EDG2 /LPA-1 peptide (ab184693).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.

    • Tissue specificity

      Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.

    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.

    • Cellular localization

      Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
    • Information by UniProt

    • Database links

      • Alternative names

        • 5031439C20 antibody
        • AI326300 antibody
        • EDG 2 antibody
        • EDG2 antibody
        • Endothelial differentiation gene 2 antibody
        • Endothelial differentiation, lysophosphatidic acid G protein coupled receptor, 2 antibody
        • GPCR 26 antibody
        • Gpcr26 antibody
        • Gpcr91 antibody
        • GPR26 antibody
        • Kdt2 antibody
        • LPA 1 antibody
        • LPA receptor 1 antibody
        • LPA receptor EDG2 antibody
        • LPA-1 antibody
        • lpA1 antibody
        • Lpar1 antibody
        • LPAR1_HUMAN antibody
        • Lysophosphatidic acid receptor 1 antibody
        • Lysophosphatidic acid receptor Edg-2 antibody
        • Lysophosphatidic acid receptor EDG2 antibody
        • MGC105279 antibody
        • MGC29102 antibody
        • Mrec1.3 antibody
        • rec.1.3 antibody
        • Ventricular zone gene 1 antibody
        • vzg-1 antibody
        • VZG1 antibody
        see all

      Images

      • Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
        Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
        All lanes : Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : Caco 2 cell lysate
        Lane 4 : A375 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : goat anti-rabbit HRP IgG antibody at 1/2000 dilution

        Predicted band size: 39 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

        Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
        Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
        Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166903 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      References

      This product has been referenced in:

      • Akoume MY  et al. A Differential Hypofunctionality of Gai Proteins Occurs in Adolescent Idiopathic Scoliosis and Correlates with the Risk of Disease Progression. Sci Rep 9:10074 (2019). Read more (PubMed: 31296888) »
      • Cha YJ & Koo JS Expression of Autotaxin?Lysophosphatidate Signaling-Related Proteins in Breast Cancer with Adipose Stroma. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 31035435) »
      See all 7 Publications for this product

      Publishing research using ab166903? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-EDG2 / LPA-1 antibody [EPR9710]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Purified protein (BEL-7404)
      Gel Running Conditions
      Non-reduced Denaturing (10%)
      Loading amount
      50 µg
      Treatment
      80 µM actinomycin for 24hrs
      Specification
      BEL-7404
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Dr. guoqing zhu

      Verified customer

      Submitted Jan 26 2016

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