For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit monoclonal [EPR4541(2)] to EDG3
ICC/IF more details Unsuitable for:
Flow Cyt or IP
Synthetic peptide corresponding to residues in Human EDG3 (Q99500).
Human kidney tissue; Human heart, HepG2 and 293T cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K
Preservative: 0.01% Sodium azide Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
Belongs to the G-protein coupled receptor 1 family.
Information by UniProt
EDG 3 antibody
Endothelial differentiation G protein coupled receptor 3 antibody
Western blot - Anti-EDG3 antibody [EPR4541(2)] (ab126622)
All lanes : Anti-EDG3 antibody [EPR4541(2)] (ab126622) at 1/1000 dilution Lane 1 : HepG2 cell lysate Lane 2 : Human heart tissue lysate Lane 3 : 293T cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3 antibody [EPR4541(2)] (ab126622)
ab126622, at a 1/50 dilution, staining EDG3 in paraffin-embedded Human kidney tissue by immunohistochemistry.
Other - Anti-EDG3 antibody [EPR4541(2)] (ab126622)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"