Product nameAnti-EDG3/S1P3 antibody [EPR4541(2)]
See all EDG3/S1P3 primary antibodies
DescriptionRabbit monoclonal [EPR4541(2)] to EDG3/S1P3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human EDG3/S1P3 aa 300-150.
Database link: Q99500
- Human kidney tissue; Human heart, HepG2 and 293T cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as EDG3.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.80 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionReceptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Tissue specificityExpressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- EDG 3 antibody
- EDG3 antibody
- Endothelial differentiation G protein coupled receptor 3 antibody
All lanes : Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human heart tissue lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
ab126622, at a 1/50 dilution, staining EDG3/S1P3 in paraffin-embedded Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Palangi A et al. Differential expression of S1P receptor subtypes in human bladder transitional cell carcinoma. Clin Transl Oncol N/A:N/A (2019). Read more (PubMed: 30712233) »
- Shen Y et al. S1P/S1PR3 axis promotes aerobic glycolysis by YAP/c-MYC/PGAM1 axis in osteosarcoma. EBioMedicine N/A:N/A (2018). Read more (PubMed: 30587459) »