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    edg6s1pr4-antibody-ab126392.pdf

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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-EDG6/S1PR4 antibody (ab126392)

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Western blot - Anti-EDG6/S1PR4 antibody (ab126392)

    Key features and details

    • Rabbit polyclonal to EDG6/S1PR4
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-EDG6/S1PR4 antibody
      See all EDG6/S1PR4 primary antibodies
    • Description

      Rabbit polyclonal to EDG6/S1PR4
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human EDG6/S1PR4 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab126640)

    • Positive control

      • This antibody gave a positive signal in both HEK293 and Jurkat whole cell lysates.
    • General notes

       This product was previously labelled as EDG6

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR
      • Signal Transduction
      • Metabolism
      • Vitamins / Minerals
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Cofactors, Vitamins / minerals
      • Vitamins / minerals
      • Metabolism
      • Types of disease
      • Cancer
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab126392 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Pig
    Macaque monkey
    Gorilla
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).

    Target

    • Function

      Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes.
    • Tissue specificity

      Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession O95977 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 101150342 Gorilla
      • Entrez Gene: 8698 Human
      • Entrez Gene: 100517997 Pig
      • Omim: 603751 Human
      • SwissProt: O95977 Human
      • Unigene: 662006 Human
      • Alternative names

        • EDG6 antibody
        • Endothelial differentiation lysophosphatidic acid G protein coupled receptor 6 antibody
        • Endothelial differentiation G protein coupled receptor 6 antibody
        • Endothelial differentiation G-protein coupled receptor 6 antibody
        • Endothelial differentiation gene 6 antibody
        • LPC1 antibody
        • Lysophospholipid receptor C1 antibody
        • MGC129298 antibody
        • Putative G protein coupled receptor antibody
        • S1P receptor 4 antibody
        • S1P receptor Edg-6 antibody
        • S1P receptor Edg6 antibody
        • S1P4 antibody
        • S1PR4 antibody
        • S1PR4_HUMAN antibody
        • SLP4 antibody
        • Sphingosine 1 phosphate receptor 4 antibody
        • Sphingosine 1 phosphate receptor Edg6 antibody
        • Sphingosine 1-phosphate receptor 4 antibody
        • Sphingosine 1-phosphate receptor Edg-6 antibody
        see all

      Images

      • Western blot - Anti-EDG6/S1PR4 antibody (ab126392)
        Western blot - Anti-EDG6/S1PR4 antibody (ab126392)
        All lanes : Anti-EDG6/S1PR4 antibody (ab126392) at 1 µg/ml

        Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
        Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 42 kDa
        Observed band size: 48 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 22 kDa, 30 kDa, 36 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 30 seconds


        EDG6/S1PR4 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab126392? Please let us know so that we can cite the reference in this datasheet.

      ab126392 has not yet been referenced specifically in any publications.

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