Anti-EDG6/S1PR4 antibody (ab126392)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-EDG6/S1PR4 antibody
See all EDG6/S1PR4 primary antibodies -
Description
Rabbit polyclonal to EDG6/S1PR4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig, Macaque monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human EDG6/S1PR4 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab126640) -
Positive control
- This antibody gave a positive signal in both HEK293 and Jurkat whole cell lysates.
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General notes
This product was previously labelled as EDG6
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab126392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa). |
Target
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Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes. -
Tissue specificity
Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 101150342 Gorilla
- Entrez Gene: 8698 Human
- Entrez Gene: 100517997 Pig
- Omim: 603751 Human
- SwissProt: O95977 Human
- Unigene: 662006 Human
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Alternative names
- EDG6 antibody
- Endothelial differentiation lysophosphatidic acid G protein coupled receptor 6 antibody
- Endothelial differentiation G protein coupled receptor 6 antibody
see all
Images
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Western blot - Anti-EDG6/S1PR4 antibody (ab126392)All lanes : Anti-EDG6/S1PR4 antibody (ab126392) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 30 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsEDG6/S1PR4 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
Datasheets and documents
References
ab126392 has not yet been referenced specifically in any publications.
