Key features and details
- Rabbit polyclonal to EDG6/S1PR4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EDG6/S1PR4 antibody
See all EDG6/S1PR4 primary antibodies
DescriptionRabbit polyclonal to EDG6/S1PR4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Pig, Macaque monkey, Gorilla
Synthetic peptide corresponding to Human EDG6/S1PR4 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Jurkat whole cell lysate as well as the following tissue lysates: Human Spleen; Human Tonsil; Human Lymph node.
This product was previously labelled as EDG6
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab92993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).|
FunctionReceptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes.
Tissue specificitySpecifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- EDG6 antibody
- Endothelial differentiation lysophosphatidic acid G protein coupled receptor 6 antibody
- Endothelial differentiation G protein coupled receptor 6 antibody
All lanes : Anti-EDG6/S1PR4 antibody (ab92993) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Tonsil (Human) Tissue Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
EDG6/S1PR4 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab92993 has not yet been referenced specifically in any publications.