Key features and details
- Rabbit polyclonal to EDG8
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EDG8 antibody
See all EDG8 primary antibodies
DescriptionRabbit polyclonal to EDG8
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
- This antibody gave a positive signal in the following lysates: Brain: corpus callosum (Human) Whole Cell Lysate; Brain (Human) Tissue Lysate; Spleen (Human) Tissue Lysate; Lung (Human) Tissue Lysate; U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate; MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab92994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 42 kDa).|
FunctionReceptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins (By similarity). May play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells.
Tissue specificityWidely expressed in the brain, most prominently in the corpus callosum, which is predominantly white matter. Detected in spleen, peripheral blood leukocytes, placenta, lung, aorta and fetal spleen. Low-level signal detected in many tissue extracts. Overexpressed in leukemic large granular lymphocytes. Isoform 1 is predominantly expressed in peripheral tissues. Isoform 2 is expressed in brain, spleen and peripheral blood leukocytes.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Developmental stageAt 24 weeks of gestation, fragments of radial glial fibers are positive within the cortical plate and subplate of allocortical areas. These positive fragments often appear enlarged as varicosities and some of them terminate at blood vessels. Between 28 and 30 weeks of gestation, all iso- and allocortical areas contain immunolabelled radial glial fibers revealing curvature next to sulci. After 32 weeks of gestation, radial glial fibers gradually disappear; instead positive transitional stages between radial glia and astrocytes were found.
Cellular localizationCell membrane.
- Information by UniProt
- EDG 8 antibody
- EDG8 S1P receptor antibody
- Endothelial differentiation G protein coupled receptor 8 antibody
All lanes : Anti-EDG8 antibody (ab92994) at 1 µg/ml
Lane 1 : Human brain corpus callosum tissue lysate (alzheimer) - total protein (ab30027)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human spleen tissue lysate - total protein (ab29699)
Lane 4 : Lung (Human) Tissue Lysate
Lane 5 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ICC/IF image of ab92994 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92994 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of EDG8 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92994, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab92994 has been referenced in 3 publications.
- Gläser A et al. Identification of Brain-Specific Treatment Effects in NPC1 Disease by Focusing on Cellular and Molecular Changes of Sphingosine-1-Phosphate Metabolism. Int J Mol Sci 21:N/A (2020). PubMed: 32599915
- Palangi A et al. Differential expression of S1P receptor subtypes in human bladder transitional cell carcinoma. Clin Transl Oncol N/A:N/A (2019). PubMed: 30712233
- Arana L et al. Ceramide 1-phosphate induces macrophage chemoattractant protein-1 release: involvement in ceramide 1-phosphate-stimulated cell migration. Am J Physiol Endocrinol Metab 304:E1213-26 (2013). PubMed: 23548612