Key features and details
- Rabbit polyclonal to EDJ
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-EDJ antibody
See all EDJ primary antibodies
DescriptionRabbit polyclonal to EDJ
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Orangutan
- WB: HeLa, NIH/3T3 and NBT-II cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human pancreas tissue.
Previously labelled as DNAJB11.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 40 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionServes as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 J domain.
modificationsContains high-mannose Endo H-sensitive carbohydrates.
Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.
Thr-188 was reported (PubMed:17525332) to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven.
Cellular localizationEndoplasmic reticulum lumen. Associated with the ER membrane in a C-terminally epitope-tagged construct.
- Information by UniProt
- ABBP-2 antibody
- ABBP2 antibody
- APOBEC1 binding protein 2 antibody
Anti-EDJ antibody (ab224082) at 1/100 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 40 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for EDJ (green) using ab224082 (4 µg/ml) in ICC/IF.
Paraffin embedded human pancreas tissue stained for EDJ with ab224082 (1/200 dilution) in immunohistochemical analysis.
All lanes : Anti-EDJ antibody (ab224082) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat cell line) cell lysate
Predicted band size: 40 kDa
ab224082 has not yet been referenced specifically in any publications.