Key features and details
- Rabbit polyclonal to EDJ
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-EDJ antibody
See all EDJ primary antibodies
DescriptionRabbit polyclonal to EDJ
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Cow, Human, Pig
Recombinant fragment within Human EDJ (internal sequence). The exact sequence is proprietary.
Database link: Q9UBS4
- IHC-P: Mouse liver tissue; rat liver and hind brain tissue.
Previously labelled as DNAJB11.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
FunctionServes as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 J domain.
modificationsContains high-mannose Endo H-sensitive carbohydrates.
Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.
Thr-188 was reported (PubMed:17525332) to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven.
Cellular localizationEndoplasmic reticulum lumen. Associated with the ER membrane in a C-terminally epitope-tagged construct.
- Information by UniProt
- ABBP-2 antibody
- ABBP2 antibody
- APOBEC1 binding protein 2 antibody
Paraffin-embedded mouse liver tissue stained for EDJ with ab226941 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat liver tissue stained for EDJ with ab226941 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat hind brain tissue stained for EDJ with ab226941 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226941 has not yet been referenced specifically in any publications.