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Anti-EDN3 antibody (ab96709)

  • Datasheet
  • SDS
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Western blot - Anti-EDN3 antibody (ab96709)

    Key features and details

    • Rabbit polyclonal to EDN3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human EDN3 protein (ab202211)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-EDN3 antibody
      See all EDN3 primary antibodies
    • Description

      Rabbit polyclonal to EDN3
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment corresponding to Human EDN3 aa 1-187.

    • Positive control

      • Raji whole cell lysate; 293T, A431, H1299, HeLa, HepG2 and Molt-4 cell lysates
    • General notes

      Previously labelled as Endothelin 3. 

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Cancer
      • Invasion/microenvironment
      • Hypoxia
      • HIF
      • Cancer
      • Signal transduction
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Raji whole cell lysate (ab30124)
    • Recombinant Protein

      • Recombinant Human EDN3 protein (ab202211)

    Applications

    Our Abpromise guarantee covers the use of ab96709 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.

    Target

    • Function

      Endothelins are endothelium-derived vasoconstrictor peptides.
    • Tissue specificity

      Expressed in trophoblasts and placental stem villi vessels, but not in cultured placental smooth muscle cells.
    • Involvement in disease

      Defects in EDN3 are the cause of Hirschsprung disease type 4 (HSCR4) [MIM:613712]; also known as aganglionic megacolon (MGC). A genetic disorder of neural crest development characterized by the absence of intramural ganglion cells in the hindgut; often resulting in intestinal obstruction.
      Defects in EDN3 are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
      Defects in EDN3 are a cause of Waardenburg syndrome type 4 (WS4B) [MIM:613265]; also known as Waardenburg-Shah syndrome. WS4B is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    • Sequence similarities

      Belongs to the endothelin/sarafotoxin family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P14138 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1908 Human
      • Omim: 131242 Human
      • SwissProt: P14138 Human
      • Unigene: 1408 Human
      • Alternative names

        • EDN3 antibody
        • EDN3_HUMAN antibody
        • Endothelin 3 antibody
        • Endothelin-3 antibody
        • ET 3 antibody
        • ET-3 antibody
        • PPET3 antibody
        • Preproendothelin 3 antibody
        • Preproendothelin-3 antibody
        see all

      Images

      • Western blot - Anti-EDN3 antibody (ab96709)
        Western blot - Anti-EDN3 antibody (ab96709)
        Anti-EDN3 antibody (ab96709) at 1/1000 dilution + Raji whole cell lysate at 30 µg

        Predicted band size: 25 kDa



        12% SDS-PAGE

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab96709? Please let us know so that we can cite the reference in this datasheet.

      ab96709 has been referenced in 1 publication.

      • Liu Y  et al. Autocrine endothelin-3/endothelin receptor B signaling maintains cellular and molecular properties of glioblastoma stem cells. Mol Cancer Res 9:1668-85 (2011). PubMed: 22013079

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