Anti-EDN3 antibody (ab96709)
Key features and details
- Rabbit polyclonal to EDN3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EDN3 antibody
See all EDN3 primary antibodies -
Description
Rabbit polyclonal to EDN3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human EDN3 aa 1-187.
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Positive control
- Raji whole cell lysate; 293T, A431, H1299, HeLa, HepG2 and Molt-4 cell lysates
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General notes
Previously labelled as Endothelin 3.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 25 kDa. |
Target
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Function
Endothelins are endothelium-derived vasoconstrictor peptides. -
Tissue specificity
Expressed in trophoblasts and placental stem villi vessels, but not in cultured placental smooth muscle cells. -
Involvement in disease
Defects in EDN3 are the cause of Hirschsprung disease type 4 (HSCR4) [MIM:613712]; also known as aganglionic megacolon (MGC). A genetic disorder of neural crest development characterized by the absence of intramural ganglion cells in the hindgut; often resulting in intestinal obstruction.
Defects in EDN3 are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
Defects in EDN3 are a cause of Waardenburg syndrome type 4 (WS4B) [MIM:613265]; also known as Waardenburg-Shah syndrome. WS4B is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). -
Sequence similarities
Belongs to the endothelin/sarafotoxin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1908 Human
- Omim: 131242 Human
- SwissProt: P14138 Human
- Unigene: 1408 Human
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Alternative names
- EDN3 antibody
- EDN3_HUMAN antibody
- Endothelin 3 antibody
see all
Images
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Western blot - Anti-EDN3 antibody (ab96709)Anti-EDN3 antibody (ab96709) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 25 kDa
12% SDS-PAGE
References (1)
ab96709 has been referenced in 1 publication.
- Liu Y et al. Autocrine endothelin-3/endothelin receptor B signaling maintains cellular and molecular properties of glioblastoma stem cells. Mol Cancer Res 9:1668-85 (2011). PubMed: 22013079
