EdU, DNA synthesis monitoring probe (ab146186)
- Datasheet
- References
Overview
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Product nameEdU, DNA synthesis monitoring probe
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DescriptionDNA synthesis monitoring probe
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Alternative names
- 2'-Deoxy-5-ethynyluridine
- 5-EdU
- 5-Ethynyl-2'-deoxyuridine
- EdU
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Biological description
Thymidine analog. Alternative to BrdU (5-Bromo-2’-deoxyuridine) (ab142567) for measuring de novo DNA synthesis during S-phase. Cytotoxic anti-metabolite. Used for probing short-term DNA synthesis in tissue culture and living organisms where prolonged cell survival is not required.
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Purity> 98%
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CAS Number61135-33-9
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Chemical structure
Properties
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Molecular weight252.22
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Molecular formulaC11H12N2O5
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Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
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Solubility overview
Soluble in DMSO (up to 10 mg/ml). Solutions in DMSO may be stored at -20°C for up to 2 months.
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILESC#Cc1cn(c(=O)[nH]c1=O)[C@H]2C[C@@H]([C@H](O2)CO)O
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Source
Synthetic
References
This product has been referenced in:
- Kohlmeier F et al. EdU induces DNA damage response and cell death in mESC in culture. Chromosome Res 21:87-100 (2013). Read more (PubMed: 23463495) »
- Kotogány E et al. A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine. Plant Methods 6:5 (2010). Read more (PubMed: 20181034) »
- Limsirichaikul S et al. A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU). Nucleic Acids Res 37:e31 (2009). Read more (PubMed: 19179371) »
- Buck SB et al. Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies. Biotechniques 44:927-9 (2008). Read more (PubMed: 18533904) »
- Salic A & Mitchison TJ A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci U S A 105:2415-20 (2008). Read more (PubMed: 18272492) »
