EdU, DNA synthesis monitoring probe (ab146186)
Overview
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Product name
EdU, DNA synthesis monitoring probe -
Description
DNA synthesis monitoring probe -
Alternative names
- 2'-Deoxy-5-ethynyluridine
- 5-EdU
- 5-Ethynyl-2'-deoxyuridine
- EdU
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Biological description
Thymidine analog. Alternative to BrdU (5-Bromo-2’-deoxyuridine) (ab142567) for measuring de novo DNA synthesis during S-phase. Cytotoxic anti-metabolite. Used for probing short-term DNA synthesis in tissue culture and living organisms where prolonged cell survival is not required.
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Purity
> 98% -
CAS Number
61135-33-9 -
Chemical structure
Properties
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Molecular weight
252.22 -
Molecular formula
C11H12N2O5 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 10 mM and in DMSO to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C#Cc1cn(c(=O)[nH]c1=O)[C@H]2C[C@@H]([C@H](O2)CO)O -
Source
Synthetic
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References
ab146186 has not yet been referenced specifically in any publications.
