Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
- Datasheet
- Specific References (42)
- Protocols
Overview
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Product nameAnti-EEA1 antibody [1G11] - Early Endosome Marker
See all EEA1 primary antibodies -
DescriptionMouse monoclonal [1G11] to EEA1 - Early Endosome Marker
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IFmore details
Unsuitable for: WB -
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit
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Immunogen
Recombinant fragment derived from within residues 50 - 150 of Human EEA1.
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Positive control
- This anitbody gave a positive immunofluorescent signal in the following cell lines: HeLa HepG2
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone number1G11
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab70521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 5 µg/ml. |
Target
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FunctionBinds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
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Sequence similaritiesContains 1 C2H2-type zinc finger.
Contains 1 FYVE-type zinc finger. -
DomainThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
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Cellular localizationCytoplasm. Early endosome membrane.
- Information by UniProt
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Database links
- Entrez Gene: 8411 Human
- Entrez Gene: 216238 Mouse
- Entrez Gene: 314764 Rat
- Omim: 605070 Human
- SwissProt: Q15075 Human
- SwissProt: Q8BL66 Mouse
- Unigene: 567367 Human
- Unigene: 210035 Mouse
see all -
Alternative names
- Early endosome antigen 1 antibody
- Early endosome antigen 1, 162kD antibody
- Early endosome associated protein antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)](https://www.abcam.com/ps/products/70/ab70521/Images/2009-01-06_004ahepp-ab70521 ap579625 edited.jpeg.jpeg)
Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)ICC/IF image of ab70521 stained HepG2 cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70521 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml. -
![Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)](https://www.abcam.com/ps/products/70/ab70521/Images/EEA1-Primary-antibodies-ab70521-12.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)ICC/IF image of ab70521 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70521, 10µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 oat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)](https://www.abcam.com/ps/products/70/ab70521/Images/EEA1-Primary-antibodies-ab70521-6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)This image is a courtesy of an anonymous abreview.ab70521 staining EEA1 in human THP-1 cells by Immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed, permeabilized in TritonX100 and blocked in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/200 (PBS + Tween20 + BSA 1%) and incubated with sample for 1 hour at 25°C. A 1.4 nm Gold (ultra-small) and Alexa Fluor® 594 conjugated goat polyclonal to mouse IgG1, diluted 1/500 was used as secondary. The red staining in image show EEA1 staining whilst green cytosolic staining corresponds to StarD5.
References
This product has been referenced in:
- Autour A et al. Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells. Nat Commun 9:656 (2018). Read more (PubMed: 29440634) »
- Puri C et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell 45:114-131.e8 (2018). WB, ICC/IF . Read more (PubMed: 29634932) »
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