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  1. Link

    eea1-antibody-1g11-early-endosome-marker-ab70521.pdf

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Neuroscience Cell Type Marker Neuron marker Soma marker
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Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)

  • Datasheet
  • SDS
Reviews (8)Q&A (1)References (64)

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Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)

Key features and details

  • Mouse monoclonal [1G11] to EEA1 - Early Endosome Marker
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
Protein
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Recombinant Human EEA1 protein (ab112379)
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Alexa Fluor® 647 Anti-RAB7 antibody [EPR7589] (ab198337)

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Overview

  • Product name

    Anti-EEA1 antibody [1G11] - Early Endosome Marker
    See all EEA1 primary antibodies
  • Description

    Mouse monoclonal [1G11] to EEA1 - Early Endosome Marker
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Recombinant fragment corresponding to Human EEA1 aa 50 to the C-terminus.

  • Positive control

    • This anitbody gave a positive immunofluorescent signal in the following cell lines: HeLa HepG2
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    1G11
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Endosome
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Regulation
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human EEA1 protein (ab112379)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (6)
Use a concentration of 1 - 5 µg/ml.
Notes
ICC/IF
Use a concentration of 1 - 5 µg/ml.
Application notes
Is unsuitable for WB.

Target

  • Function

    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 FYVE-type zinc finger.
  • Domain

    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Cellular localization

    Cytoplasm. Early endosome membrane.
  • Target information above from: UniProt accession Q15075 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8411 Human
    • Entrez Gene: 216238 Mouse
    • Entrez Gene: 314764 Rat
    • Omim: 605070 Human
    • SwissProt: Q15075 Human
    • SwissProt: Q8BL66 Mouse
    • Unigene: 567367 Human
    • Unigene: 210035 Mouse
    • Alternative names

      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
      Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)

      ICC/IF image of ab70521 stained HepG2 cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70521 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.

    • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
      Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)

      ICC/IF image of ab70521 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70521, 10µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 oat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
      Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)This image is a courtesy of an anonymous abreview.

      ab70521 staining EEA1 in human THP-1 cells by Immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed, permeabilized in TritonX100 and blocked in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/200 (PBS + Tween20 + BSA 1%) and incubated with sample for 1 hour at 25°C. A 1.4 nm Gold (ultra-small) and Alexa Fluor® 594 conjugated goat polyclonal to mouse IgG1, diluted 1/500 was used as secondary. The red staining in image show EEA1 staining whilst green cytosolic staining corresponds to StarD5.

      See Abreview

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (64)

    Publishing research using ab70521? Please let us know so that we can cite the reference in this datasheet.

    ab70521 has been referenced in 64 publications.

    • Saveri P  et al. Charcot-Marie-Tooth Type 2B: A New Phenotype Associated with a Novel RAB7A Mutation and Inhibited EGFR Degradation. Cells 9:N/A (2020). PubMed: 32326241
    • Taverner A  et al. Cholix protein domain I functions as a carrier element for efficient apical to basal epithelial transcytosis. Tissue Barriers 8:1710429 (2020). PubMed: 31928299
    • Yan P  et al. Molecular Stressors Engender Protein Connectivity Dysfunction through Aberrant N-Glycosylation of a Chaperone. Cell Rep 31:107840 (2020). PubMed: 32610141
    • Gao M  et al. Poldip2 mediates blood-brain barrier disruption and cerebral edema by inducing AQP4 polarity loss in mouse bacterial meningitis model. CNS Neurosci Ther 26:1288-1302 (2020). PubMed: 32790044
    • Fay NC  et al. A Novel Fusion of IL-10 Engineered to Traffic across Intestinal Epithelium to Treat Colitis. J Immunol 205:3191-3204 (2020). PubMed: 33148717
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody [1G11] - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Foreskin Fibroblasts (HFF))
    Permeabilization
    Yes - Triton X-100
    Specification
    Human Foreskin Fibroblasts (HFF)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 11 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody [1G11] - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (Epithelial intestinal cell line)
    Permeabilization
    Yes - 0.1% Tween
    Specification
    Epithelial intestinal cell line
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Sébastien Holbert

    Verified customer

    Submitted Aug 22 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody [1G11] - Early Endosome Marker

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 24°C
    Sample
    Monkey Cell (Kidney)
    Specification
    Kidney
    Permeabilization
    Yes - TritonX100 al 0,1%, Tween20
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 24 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody [1G11] - Early Endosome Marker

    Poor
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample
    Mouse Cell (hippocampal neuronal culture)
    Specification
    hippocampal neuronal culture
    Permeabilization
    Yes - 0.2% Triton
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 12 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody [1G11] - Early Endosome Marker

    Poor
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Guinea pig Cell (distal colon)
    Specification
    distal colon
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 19 2010

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-EEA1 antibody [1G11] - Early Endosome Marker

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Read More

    DR. Sophie Pezet

    Verified customer

    Submitted Sep 08 2010

    Immunocytochemistry abreview for Anti-EEA1 antibody [1G11] - Early Endosome Marker

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (primary hippocampal neurons)
    Specification
    primary hippocampal neurons
    Fixative
    4% PFA/Sucrose
    Permeabilization
    Yes - 0,2% Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 10 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody [1G11] - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (THP-1)
    Specification
    THP-1
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 01 2010

    Question

    Dear Sir/ Madam
    I want to use ab70521 as the primary antibody to mark early endosome.
    Could you please recommend me a secondary antibody which is suitable for
    the primary antibody. I need it to be green fluorescence labeled. Tanks a
    lot.
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 08 2012

    Answer

    Thank you for your inquiry.

    We have two secondary antibodies that match this mouse IgG1 primary antibody:

    ab97239:

    https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-ab97239.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-ab97239.html).

    ab98692:

    https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-preadsorbed-ab98692.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-preadsorbed-ab98692.html).

    Both antibodies are tested and guaranteed for Flow Cyt, ICC/IF and IHC.

    I hope this information was helpful and wish you good luck for your experiments.

    Read More

    Abcam Scientific Support

    Answered on Mar 08 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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