Overview

  • Product name

  • Description

    Goat polyclonal to EEA1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human EEA1 aa 821-835 (internal sequence). NP_003557.2.
    Sequence:

    QETKIQHEELNNRIQ


    Database link: Q15075

  • Positive control

    • A549 and HEK293 lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab206860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 FYVE-type zinc finger.
  • Domain

    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Cellular localization

    Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Early endosome antigen 1 antibody
    • Early endosome antigen 1, 162kD antibody
    • Early endosome associated protein antibody
    • EEA 1 antibody
    • EEA1 antibody
    • EEA1_HUMAN antibody
    • Endosome associated protein p162 antibody
    • Endosome-associated protein p162 antibody
    • MST105 antibody
    • MSTP105 antibody
    • ZFYVE2 antibody
    • Zinc finger FYVE domain containing protein 2 antibody
    • Zinc finger FYVE domain-containing protein 2 antibody
    see all

Images

  • Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells permeabilized with 0.15% Triton staining EEA1. Primary incubation with ab206860 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.
  • Anti-EEA1 antibody (ab206860) at 0.3 µg/ml + HEK293 lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 162 kDa

References

ab206860 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab206860.
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