Overview

  • Product name
  • Description
    Rabbit polyclonal to EEA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    GSQGSDLDSSATPINTVDVNNESSSEG

    conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 24 - 40 of Human EEA1

  • Positive control
    • whole extract of mouse NIH-3T3 cells; human HeLa and rat NRK cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab50313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.4 - 0.8 µg/ml. Detects a band of approximately 160 kDa.
ICC Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • Function
    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 FYVE-type zinc finger.
  • Domain
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Cellular localization
    Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Early endosome antigen 1 antibody
    • Early endosome antigen 1, 162kD antibody
    • Early endosome associated protein antibody
    • EEA 1 antibody
    • EEA1 antibody
    • EEA1_HUMAN antibody
    • Endosome associated protein p162 antibody
    • Endosome-associated protein p162 antibody
    • MST105 antibody
    • MSTP105 antibody
    • ZFYVE2 antibody
    • Zinc finger FYVE domain containing protein 2 antibody
    • Zinc finger FYVE domain-containing protein 2 antibody
    see all

Images

  • HeLa cells fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 µg/mL ab50313."
  • ab50313 staining EEA1 in mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 250C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3®-conjugated goat polyclonal to rabbit IgG (H&L) was used as secondary antibody at 1/200 dilution.

    See Abreview

References

This product has been referenced in:
  • Leiva-Rodríguez T  et al. ATG5 overexpression is neuroprotective and attenuates cytoskeletal and vesicle-trafficking alterations in axotomized motoneurons. Cell Death Dis 9:626 (2018). Read more (PubMed: 29799519) »
  • Acharya M  et al. av Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells. Nat Commun 7:10917 (2016). Read more (PubMed: 26965188) »

See all 12 Publications for this product

Customer reviews and Q&As

Thank you very much for your responses.
I'm sending a free of charge vial of ab95195 on the order ***, which should arrive tomorrow. Please keep me updated about the results using this antibody.
For the replacement of ab50313, I certainly und...

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Thank you very much for your reply and for sending all of this information.
I will be happy to send the replacement ab95195, but I will need the original order number or PO number that ab2074 was received on. Would you be able to provide this info...

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Thanks for the update. We certainly appreciate your lab's troubleshooting efforts. Please feel free to reply after your trip.

We have previously been in contact regarding ab2074, which was not providing satisfactory results. We had offered a free of charge replacement with a different antibody, but we were waiting to hear from you with your selection. If you have dete...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (primary astrocyte)
Specification
primary astrocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% saponin
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 08 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Lung carcinoma cell line)
Specification
Lung carcinoma cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Aug 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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