- Datasheet
- Specific References (12)
- Protocols
Overview
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Product nameAnti-EEA1 antibody
See all EEA1 primary antibodies -
DescriptionRabbit polyclonal to EEA1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide:
GSQGSDLDSSATPINTVDVNNESSSEG
conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 24 - 40 of Human EEA1 -
Positive control
- whole extract of mouse NIH-3T3 cells; human HeLa and rat NRK cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab50313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.4 - 0.8 µg/ml. Detects a band of approximately 160 kDa. | |
| ICC | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 - 10 µg/ml. |
Target
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FunctionBinds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
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Sequence similaritiesContains 1 C2H2-type zinc finger.
Contains 1 FYVE-type zinc finger. -
DomainThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
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Cellular localizationCytoplasm. Early endosome membrane.
- Information by UniProt
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Database links
- Entrez Gene: 8411 Human
- Entrez Gene: 216238 Mouse
- Entrez Gene: 314764 Rat
- Omim: 605070 Human
- SwissProt: Q15075 Human
- SwissProt: Q8BL66 Mouse
- Unigene: 567367 Human
- Unigene: 210035 Mouse
see all -
Alternative names
- Early endosome antigen 1 antibody
- Early endosome antigen 1, 162kD antibody
- Early endosome associated protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody (ab50313)HeLa cells fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 µg/mL ab50313." -
Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody (ab50313)This image is a courtesy of Anonymous Abreviewab50313 staining EEA1 in mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 250C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3®-conjugated goat polyclonal to rabbit IgG (H&L) was used as secondary antibody at 1/200 dilution.
References
This product has been referenced in:
- Leiva-Rodríguez T et al. ATG5 overexpression is neuroprotective and attenuates cytoskeletal and vesicle-trafficking alterations in axotomized motoneurons. Cell Death Dis 9:626 (2018). Read more (PubMed: 29799519) »
- Acharya M et al. av Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells. Nat Commun 7:10917 (2016). Read more (PubMed: 26965188) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"