Anti-EEA1 antibody - Early Endosome Marker (ab206860)
Key features and details
- Goat polyclonal to EEA1 - Early Endosome Marker
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EEA1 antibody - Early Endosome Marker
See all EEA1 primary antibodies -
Description
Goat polyclonal to EEA1 - Early Endosome Marker -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human EEA1 aa 800-900 (internal sequence). NP_003557.2.
Database link: Q15075 -
Positive control
- A549 and HEK293 lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab206860 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
1 hour primary incubation is recommended for this product. |
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ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa). 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 FYVE-type zinc finger. -
Domain
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions. -
Cellular localization
Cytoplasm. Early endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 8411 Human
- Entrez Gene: 216238 Mouse
- Omim: 605070 Human
- SwissProt: Q15075 Human
- SwissProt: Q8BL66 Mouse
- Unigene: 567367 Human
- Unigene: 210035 Mouse
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Alternative names
- Early endosome antigen 1 antibody
- Early endosome antigen 1, 162kD antibody
- Early endosome associated protein antibody
see all
Images
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Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells permeabilized with 0.15% Triton staining EEA1. Primary incubation with ab206860 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.
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Anti-EEA1 antibody - Early Endosome Marker (ab206860) at 0.3 µg/ml + HEK293 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 162 kDa
Datasheets and documents
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Datasheet download
References (4)
ab206860 has been referenced in 4 publications.
- Reißing J et al. Immunomodulatory receptor VSIG4 is released during spontaneous bacterial peritonitis and predicts short-term mortality. JHEP Rep 4:100391 (2022). PubMed: 34917912
- Bai N et al. Inhibition of SIRT2 promotes APP acetylation and ameliorates cognitive impairment in APP/PS1 transgenic mice. Cell Rep 40:111062 (2022). PubMed: 35830807
- Duchesne C et al. Cold Atmospheric Plasma Promotes Killing of Staphylococcus aureus by Macrophages. mSphere N/A:e0021721 (2021). PubMed: 34133202
- Alonso-Valenteen F et al. HER3-targeted protein chimera forms endosomolytic capsomeres and self-assembles into stealth nucleocapsids for systemic tumor homing of RNA interference in vivo. Nucleic Acids Res 47:11020-11043 (2019). PubMed: 31617560