The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Antigen retrieval (Tris-EDTA buffer) recommended.
Use at an assay dependent concentration.
1/75 - 1/600.
Antigen Retrieval: 20 min ER2 (Tris-EDTA)
Is unsuitable for IHC-Fr or WB.
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
Belongs to the WD repeat ESC family. Contains 7 WD repeats.
Expression peaks at the G1/S phase boundary.
Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
Immunohistochemical analysis of paraffin embedded Human lymph node (top left), thymus (top right), testicle (bottom left) and bone marrow (bottom right) tissues labeling EED with ab126542 antibody at 1/600 dilution.
Immunohistochemical analysis of on frozen cytospins preparations of V5-tagged Human EED expressed in Hek293T labeling EED with ab126542 antibody (left). Cytospin preparation of V5-tagged Human MNDA protein was used as a negative control (middle). Labeling with an anti-V5 mAb confirmed the efficiency of transfection (right).
Cho YJ et al. Prognostic implications of polycomb proteins ezh2, suz12, and eed1 and histone modification by H3K27me3 in sarcoma. BMC Cancer18:158 (2018).
Read more (PubMed: 29415665) »
Li J et al. EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia. Nat Neurosci16:1745-53 (2013).
Read more (PubMed: 24162653) »