Anti-EED antibody (ab254569)
Key features and details
- Rabbit polyclonal to EED
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EED antibody
See all EED primary antibodies -
Description
Rabbit polyclonal to EED -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chicken, Cow
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Immunogen
Synthetic peptide within Human EED aa 30-80. The exact sequence is proprietary. 18 amino acids long.
Database link: O75530 -
Positive control
- WB: Human heart and testis tissue lysate. ICC/IF: Human heart tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
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ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. -
Tissue specificity
Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer. -
Sequence similarities
Belongs to the WD repeat ESC family.
Contains 7 WD repeats. -
Developmental stage
Expression peaks at the G1/S phase boundary. -
Cellular localization
Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 426381 Chicken
- Entrez Gene: 404183 Cow
- Entrez Gene: 8726 Human
- Omim: 605984 Human
- SwissProt: Q5ZKH3 Chicken
- SwissProt: Q3SZ25 Cow
- SwissProt: O75530 Human
- Unigene: 503510 Human
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Alternative names
- eed antibody
- EED protein antibody
- EED, mouse, homolog of antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EED antibody (ab254569)
Immunocytochemistry/immunofluorescent analysis of human heart tissue labeling EED with ab254569 at 20µg/ml.
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Western blot - Anti-EED antibody (ab254569)Anti-EED antibody (ab254569) at 2 µg/ml + Human testis tissue lysate
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted? -
Western blot - Anti-EED antibody (ab254569)Lane 1 : Anti-EED antibody (ab254569) at 1 µg/ml
Lane 2 : Anti-EED antibody (ab254569) at 2 µg/ml
All lanes : Human heart tissue lysate
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab254569 has not yet been referenced specifically in any publications.
