Western blot abreview for Anti-EED antibody

Inconclusive
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (Extra Embryonic Endoderm Cells (XEN))
Specification
Extra Embryonic Endoderm Cells (XEN)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/200

Secondary antibody

Name
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 0 kDa Non-specific: ~50, 60, 65, 110, >1 kDa
Exposure
3 minute(s) and 0 second(s)
Negative control
EED homozygous mutant XEN cells
Positive control
wild-type XEN cell lysate, wild-type TS cell lysate

Additional data

Additional Notes
Western blot lanes: 1. EED fl/- XEN cells 2. EED -/- XEN 3. EED -/- XEN 4. WT XEN 5. WT XEN 6. WT XEN 7. WT XEN 8. WT TS (trophoblast stem cells) All bands appear to be non-specific, they are present in each lane, even the negative control EED mutant cell lines. All bands were larger than the predicted size.
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.

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Verified customer

Submitted Nov 27 2013

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