Western blot abreview for Anti-EED antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (DLD1)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
DLD1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST
Dilution
1/500

Secondary antibody

Dilution
1/10000
Name

Detection

Detection method
ECL Prime
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 70 kDa Non-specific: 80 kDa
Positive control
2

Additional data

PubMed ID
27980680: View

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Submitted Feb 02 2017

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