Western blot abreview for Anti-EED antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (THP1)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
30 µg
Specification
THP1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 100 kDa

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Submitted Sep 06 2017

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