Application
Western blot
Sample
Mouse Cell lysate - other (mouse Embryonic Stem Cells)
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-12%)
Loading amount
30 µg
Specification
mouse Embryonic Stem Cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime
Negative control
KO1, KO2 mESCs
Exposure
30 second(s)
Bands
Specific: 40, 47, 55, 60, 65 kDa Non-specific: 55, 70, 100, (120?), kDa
Positive control
WT mESCs
Additional data
Additional Notes
Few unspecific bands in the cytoplasmic fraction. Very specific in the chromatin fraction
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ivano Mocavini
Verified customer
Submitted Jan 20 2021