Recombinant Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9470] (ab140632)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9470] to eEF1A1/EF-Tu+eEF1A1 + eEF1AL3
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9470]
See all eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 primary antibodies -
Description
Rabbit monoclonal [EPR9470] to eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and 293T cell lysates. GST tagged Recombinant Human EEF1A1 and EEF1AL3 protein. ICC/IF: MCF-7 cells. IHC-P: Human brain and human breast carcinoma tissues. Flow Cyt (intra): MCF-7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9470 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/500.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa. |
Target
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Cellular localization
eEF1A1/EF-Tu: Cytoplasm. -
Database links
- Entrez Gene: 1915 Human
- Entrez Gene: 13627 Mouse
- Entrez Gene: 171361 Rat
- Omim: 130590 Human
- SwissProt: P68104 Human
- SwissProt: P10126 Mouse
- SwissProt: P62630 Rat
- Unigene: 520703 Human
see all
Images
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All lanes : Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9470] (ab140632) at 1/1000 dilution
Lane 1 : GST tagged Recombinant Human EEF1A1 protein (Full length, 76 KDa)
Lane 2 : His tagged Recombinant Human EEF1AL3 protein (Full length, 52 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 50 kDa -
All lanes : Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9470] (ab140632) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 50 kDa -
Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling eEF1A1/EF-Tu with purified ab140632 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Control: PBS only.
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Immunohistochemical analysis of paraffin embedded Human brain tissue labelling eEF1A1/EF-Tu with ab140632 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling eEF1A1/EF-Tu with ab140632 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Overlay histogram showing MCF7 cells stained with ab140632 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140632, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab140632 has been referenced in 3 publications.
- Xiang L et al. FOXQ1 promotes the osteogenic differentiation of bone mesenchymal stem cells via Wnt/ß-catenin signaling by binding with ANXA2. Stem Cell Res Ther 11:403 (2020). PubMed: 32943107
- Ezzoukhry Z et al. Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation. Nat Commun 9:2031 (2018). PubMed: 29795195
- Shao S et al. Decoding Mammalian Ribosome-mRNA States by Translational GTPase Complexes. Cell 167:1229-1240.e15 (2016). PubMed: 27863242