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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs
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RecombinantRabMAb

Recombinant Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)

  • Datasheet
  • SDS
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Western blot - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
  • Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
  • Flow Cytometry - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
  • Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9470] to eEF1A1/EF-Tu
  • Suitable for: Flow Cyt, IHC-P, ICC/IF, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-eEF1A1/EF-Tu antibody [EPR9470]
    See all eEF1A1/EF-Tu primary antibodies
  • Description

    Rabbit monoclonal [EPR9470] to eEF1A1/EF-Tu
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IF, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human eEF1A1/EF-Tu aa 300-400. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and 293T cell lysates. ICC/IF: MCF-7 cells. IHC-P: Human brain and human breast carcinoma tissues. Flow Cyt: MCF-7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9470
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome

Associated products

  • Alternative Versions

    • Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    • Alexa Fluor® 488 Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab202762)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human eEF1A1/EF-Tu protein (ab81792)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/500.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
Notes
Flow Cyt
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/500.
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
    • Tissue specificity

      Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.
    • Sequence similarities

      Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
    • Post-translational
      modifications

      ISGylated.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P68104 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1915 Human
      • Entrez Gene: 13627 Mouse
      • Entrez Gene: 171361 Rat
      • Omim: 130590 Human
      • SwissProt: P68104 Human
      • SwissProt: P10126 Mouse
      • SwissProt: P62630 Rat
      • Unigene: 520703 Human
      • Unigene: 535192 Human
      • Unigene: 586423 Human
      • Unigene: 695681 Human
      • Unigene: 713109 Human
      • Unigene: 745122 Human
      • Unigene: 138471 Mouse
      • Unigene: 335315 Mouse
      • Unigene: 360075 Mouse
      • Unigene: 391071 Mouse
      • Unigene: 468942 Mouse
      • Unigene: 144660 Rat
      • Unigene: 965 Rat
      see all
    • Alternative names

      • CCS 3 antibody
      • CCS3 antibody
      • Cervical cancer suppressor 3 antibody
      • chunp6927 antibody
      • CTCL tumor antigen antibody
      • EE1A1 antibody
      • EEF 1 antibody
      • EEF1A antibody
      • eEF1A-1 antibody
      • EEF1A1 antibody
      • EF-1-alpha-1 antibody
      • EF-Tu antibody
      • EF1A antibody
      • EF1a like protein antibody
      • EF1A1_HUMAN antibody
      • Elongation factor 1 alpha subunit antibody
      • Elongation factor 1-alpha 1 antibody
      • Elongation factor Tu antibody
      • Eukaryotic elongation factor 1 A-1 antibody
      • Eukaryotic translation elongation factor 1 alpha 1 antibody
      • Eukaryotic translation elongation factor 1 alpha 1 like 14 antibody
      • Glucocorticoid receptor AF 1 specific elongation factor antibody
      • GRAF 1EF antibody
      • HNGC:16303 antibody
      • ik:tdsubc_2a3 antibody
      • ik:tdsubc_2b3 antibody
      • LENG7 antibody
      • Leukocyte receptor cluster (LRC) member 7 antibody
      • Leukocyte receptor cluster member 7 antibody
      • Prostate tumor inducing protein 1 antibody
      • PTI1 antibody
      • tdsubc_2a3 antibody
      • Translation elongation factor 1 alpha 1 like 14 antibody
      • wu:fa91c07 antibody
      • wu:fa94b03 antibody
      • wu:fi13b09 antibody
      • xx:tdsubc_2a3 antibody
      • xx:tdsubc_2b3 antibody
      see all

    Images

    • Western blot - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
      Western blot - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
      All lanes : Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled
      Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 50 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
      Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)

      Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling eEF1A1/EF-Tu with purified ab140632 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Control: PBS only.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)

      Immunohistochemical analysis of paraffin embedded Human brain tissue labelling eEF1A1/EF-Tu with ab140632 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)

      Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling eEF1A1/EF-Tu with ab140632 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
      Flow Cytometry - Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)

      Overlay histogram showing MCF7 cells stained with ab140632 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140632, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)
      Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab140632? Please let us know so that we can cite the reference in this datasheet.

    ab140632 has been referenced in 3 publications.

    • Xiang L  et al. FOXQ1 promotes the osteogenic differentiation of bone mesenchymal stem cells via Wnt/ß-catenin signaling by binding with ANXA2. Stem Cell Res Ther 11:403 (2020). PubMed: 32943107
    • Ezzoukhry Z  et al. Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation. Nat Commun 9:2031 (2018). PubMed: 29795195
    • Shao S  et al. Decoding Mammalian Ribosome-mRNA States by Translational GTPase Complexes. Cell 167:1229-1240.e15 (2016). PubMed: 27863242

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-eEF1A1 / EF-Tu antibody [EPR9470]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Treatment
    alpha synuclein
    Specification
    human
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Ms. Sehee Oh

    Verified customer

    Submitted Jul 13 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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