Recombinant
RabMAb

Recombinant Anti-eEF1A1/EF-Tu antibody [EPR9470] (Alexa Fluor® 488) (ab202762)

Overview

  • Product name

    Anti-eEF1A1/EF-Tu antibody [EPR9470] (Alexa Fluor® 488)
    See all eEF1A1/EF-Tu primary antibodies
  • Description

    Rabbit monoclonal [EPR9470] to eEF1A1/EF-Tu (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human eEF1A1/EF-Tu aa 300-400. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab202762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/200.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
  • Tissue specificity

    Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.
  • Sequence similarities

    Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCS 3 antibody
    • CCS3 antibody
    • Cervical cancer suppressor 3 antibody
    • chunp6927 antibody
    • CTCL tumor antigen antibody
    • EE1A1 antibody
    • EEF 1 antibody
    • EEF1A antibody
    • eEF1A-1 antibody
    • EEF1A1 antibody
    • EF-1-alpha-1 antibody
    • EF-Tu antibody
    • EF1A antibody
    • EF1a like protein antibody
    • EF1A1_HUMAN antibody
    • Elongation factor 1 alpha subunit antibody
    • Elongation factor 1-alpha 1 antibody
    • Elongation factor Tu antibody
    • Eukaryotic elongation factor 1 A-1 antibody
    • Eukaryotic translation elongation factor 1 alpha 1 antibody
    • Eukaryotic translation elongation factor 1 alpha 1 like 14 antibody
    • Glucocorticoid receptor AF 1 specific elongation factor antibody
    • GRAF 1EF antibody
    • HNGC:16303 antibody
    • ik:tdsubc_2a3 antibody
    • ik:tdsubc_2b3 antibody
    • LENG7 antibody
    • Leukocyte receptor cluster (LRC) member 7 antibody
    • Leukocyte receptor cluster member 7 antibody
    • Prostate tumor inducing protein 1 antibody
    • PTI1 antibody
    • tdsubc_2a3 antibody
    • Translation elongation factor 1 alpha 1 like 14 antibody
    • wu:fa91c07 antibody
    • wu:fa94b03 antibody
    • wu:fi13b09 antibody
    • xx:tdsubc_2a3 antibody
    • xx:tdsubc_2b3 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab202762 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab202762, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ab202762 staining eEF1A1/EF-Tu in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202762 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

References

ab202762 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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