Recombinant Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] - BSA and Azide free (ab240142)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9471] to eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra), ICC/IF, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] - BSA and Azide free
See all eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 primary antibodies -
Description
Rabbit monoclonal [EPR9471] to eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 6 continuous identical amino acids with eEF1A2. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applications
Suitable for: WB, Flow Cyt (Intra), ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- GST tagged Recombinant Human EEF1A1 and EEF1AL3 protein
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General notes
ab240142 is the carrier-free version of ab157455.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9471 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] (ab157455)
- PE Anti-eEF1A1 / EF-Tu antibody [EPR9471] (ab305820)
- APC Anti-eEF1A1 / EF-Tu antibody [EPR9471] (ab305821)
- HRP Anti-eEF1A1 / EF-Tu antibody [EPR9471] (ab305822)
- Alexa Fluor® 488 Anti-eEF1A1 / EF-Tu antibody [EPR9471] (ab309690)
- Alexa Fluor® 647 Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] (ab310056)
- Alexa Fluor® 594 Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] (ab310448)
- Alexa Fluor® 555 Anti-eEF1A1 / EF-Tu antibody [EPR9471] (ab311975)
- Alexa Fluor® 568 Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] (ab312449)
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Cellular localization
eEF1A1/EF-Tu: Cytoplasm. -
Database links
- Entrez Gene: 1915 Human
- Entrez Gene: 13627 Mouse
- Entrez Gene: 171361 Rat
- Omim: 130590 Human
- SwissProt: P68104 Human
- SwissProt: P10126 Mouse
- SwissProt: P62630 Rat
- Unigene: 520703 Human
see all
Images
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All lanes : Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] (ab157455) at 1/1000 dilution
Lane 1 : GST tagged Recombinant Human EEF1A1 protein (Full length, 76 KDa)
Lane 2 : His tagged Recombinant Human EEF1AL3 protein (Full length, 52 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 50 kDaExposure time:
Lane 1: 180 seconds
Lane 2: 5 seconds
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling eEF1A1/EF-Tu with purified ab157455 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157455).
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ab157455 (purified) at 1/30 immunoprecipitating eEF1A1/EF-Tu in HeLa whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) was used for detection at 1/1,500 dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157455).
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Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling eEF1A1/EF-Tu with purified ab157455 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157455).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling eEF1A1/EF-Tu with purified ab157455 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157455).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240142 has not yet been referenced specifically in any publications.