Overview

  • Product name

  • Description

    Rabbit polyclonal to EEF1A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Positive control

    • WB: Mouse and rat brain tissue lysate; HEK-293T, A431, NCI-H1299, HeLa S3, HepG2, MOLT-4 and Raji whole cell lysate. IHC-P: Mouse and rat fore brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/10000. Predicted molecular weight: 50 kDa.

Target

  • Function

    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
  • Tissue specificity

    Brain, heart, and skeletal muscle.
  • Sequence similarities

    Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • eEF1A-2 antibody
    • Eef1a2 antibody
    • EEF1AL antibody
    • EF 1 alpha 2 antibody
    • EF-1-alpha-2 antibody
    • EF1A antibody
    • EF1A2_HUMAN antibody
    • Elongation factor 1 A 2 antibody
    • Elongation factor 1 alpha antibody
    • Elongation factor 1 alpha 2 antibody
    • Elongation factor 1-alpha 2 antibody
    • Eukaryotic elongation factor 1 A-2 antibody
    • Eukaryotic translation elongation factor 1 alpha 2 antibody
    • FLJ41696 antibody
    • HS1 antibody
    • OTTHUMP00000031776 antibody
    • Statin like antibody
    • Statin S1 antibody
    • Statin-S1 antibody
    • STN antibody
    • STNL antibody
    see all

Images

  • All lanes : Anti-EEF1A2 antibody (ab227275) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 4 : HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 6 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 7 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • Paraffin-embedded rat fore brain stained for EEF1A2 with ab227275 at 1/500 dilution in immunohistochemical analysis.

  • Anti-EEF1A2 antibody (ab227275) at 1/5000 dilution + Mouse brain tissue lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • Paraffin-embedded mouse fore brain stained for EEF1A2 with ab227275 at 1/500 dilution in immunohistochemical analysis.

  • Anti-EEF1A2 antibody (ab227275) at 1/1000 dilution + Rat brain tissue lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

References

ab227275 has not yet been referenced specifically in any publications.

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